ISSN:
1435-5604
Keywords:
Bone
;
Osteoclast
;
Tartrate-sensitive acid phospatase
;
Tartrate-resistant acid phosphatase
;
Fluoride-resistant acid phosphatase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The histochemical distribution of acid phosphatase activity in chicken tibial metaphyses was investigated with the azo-dye method, using naphthol AS-BI phosphate as a substrate, and the lead-salt method, usingβ-glycerophosphate, p-nitrophenylphosphate or adenosine triphosphate as substrates. Tartrate-resistant activity was found in cartilage and bone matrices and in osteoclasts when naphthol AS-BI phosphate, p-nitrophenylphosphate or adenosine triphosphate were used. Fluoride-resistant activity was observed in the cytoplasm of osteoclasts with naphthol AS-BI phosphate or p-nitrophenylphosphate; this activity was also insensitive to tartrate. The tartrate-resistant acid adenosine triphosphatase activity, which is due to purple acid phosphatse (type V acid phosphatase isoenzyme), was significantly weaker in the cytoplasm of osteoclasts than the tartrate-resistant acid phosphatase (TRAP) activity with naphthol AS-BI phosphate or p-nitrophenylphosphate as substrates. Furthermore, the purple acid phosphatase activity was strongly inhibited by fluoride. Therefore, the TRAP activity detected with naphthol AS-BI phosphate or p-nitrophenylphosphate may be due to the combined activity of the purple acid phosphatase and another isoenzyme, which is termed fluoride-resistant acid phosphatase (FRAP).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02377988
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