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  • Freeze substitution  (2)
  • Water expulsion vacuole  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Observations of the water expulsion vacoule ofPhytophthora palmivora (1989)
    Springer
    Protoplasma 149 (1989), S. 47-56 
    Details
    ISSN: 1615-6102
    Keywords: Phytophthora palmivora ; Water expulsion vacuole ; Coated vesicles ; Freeze substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The water expulsion vacuole ofPhytophthora palmivora was studied with the light microscope after the motility of the spores had been slowed by using a low Ca2 + medium. For transmission electron microscopy, the zoospores were prepared using freeze substitution. The water expulsion vacuole consisted of a central vacuole that collapsed and emptied its contents to the external medium. The central vacuole was surrounded by a series of interconnected vacuoles that are called the surrounding vacuole. The surrounding vacuole and the central vacuole were always associated with coated pits. The vesicles found in the region between the central and surrounding vacuole were exclusively coated vesicles. Consistent changes in the organization of the water expulsion vacuole led us to conclude that, as the central vacuole collapses, the surrounding vacuole is transformed and becomes the new central vacuole. No evidence was obtained for contraction of the central vacuole. The processes involved in membrane recycling during the functioning of the water expulsion vacuole are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01623982
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural aspects of the hyphal tip ofSclerotium rolfsii preserved by freeze substitution (1988)
    Springer
    Protoplasma 146 (1988), S. 143-149 
    Details
    ISSN: 1615-6102
    Keywords: Hyphal tip ; Ultrastructure ; Freeze substitution ; Sclerotium rolfsii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hyphal tip ofSclerotium rolfsii was examined after fixation by freeze substitution. The Spitzenkörper consisted of a dense mass of apical vesicles and microvesicles surrounding a vesicle-free zone. Linear arrangements of microvesicles were occasionally observed within the Spitzenkörper. Abundant microfilaments were seen within the Spitzenkörper region, often in close association with apical vesicles and microvesicles. Microtubules passed through the Spitzenkörper and terminated at the plasmalemma at the extreme hyphal apex. Filasomes were mostly observed within the apical region and were in close proximity to the plasmalemma. Rough ER, mitochondria, microtubules, and vacuoles were abundant in the subapical region and were usually oriented parallel to the long axis of the hypha. Ribosomes were aligned on the outer surfaces of mitochondria. Golgi body equivalents were observed throughout the subapical region and appeared as inflated cisternae of varying shapes and electron opacities. Relationships to other basidiomycetous hyphal tip cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01405923
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    Paper (German National Licenses)
    Fulltext
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