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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 40 (1999), S. 911-920 
    ISSN: 1573-5028
    Keywords: environmental stress ; homeostasis ; metabolic regulation ; seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The unique capability of rice (Oryza sativa L.) seedlings to grow under anoxic conditions may result in part from their ability to express α-amylase and maintain the supply of sugar needed for energy metabolism. Previous studies have demonstrated that under aerobic conditions the Amy1 and Amy2 subfamily genes are regulated primarily by phytohormones while the Amy3 subfamily genes are induced during sugar starvation. The expression patterns for these α-amylase genes were considerably different in anoxic vs. aerobic rice seedlings. The level of total α-amylase mRNA under anoxic conditions was decreased in aleurone layers while it increased in the embryo. Anoxic conditions greatly diminished the expression of the Amy1A gene in aleurone. Conversely, expression of many Amy3 subfamily genes was up-regulated and prolonged in embryo tissues under anoxic conditions.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: cereal seedling ; environmental stress ; G box ; signal metabolite hypothesis ; source/sink ; transgenic cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of α-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice α-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identify cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.
    Type of Medium: Electronic Resource
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