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  • 1
    Electronic Resource
    Electronic Resource
    Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues (2000)
    Springer
    Plant cell reports 19 (2000), S. 1084-1089 
    Details
    ISSN: 1432-203X
    Keywords: Key words Festuca arundinacea Schreb. ; Festuca rubra L. ; Transformation ; Highly regenerative tissue ; Transgene expression ; Abbreviations2,4-D: 2,4-Dichlorophenoxyaceticacid ; BAP: 6-Benzylaminopurine ; PAT: Phosphinothricin acetyltransferase ; GUS: β-Glucuronidase ; GFP: Green fluorescent protein gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Highly regenerative tissues of tall fescue and red fescue produced from mature seed-derived embryogenic callus were induced and proliferated on medium containing 2,4-dichlorophenoxyacetic acid (4.5 or 9.0 μM), 6-benzylaminopurine (0, 0.044, 0.44 or 2.2 μM) and cupric sulfate (0.1 or 5.0 μM) under dim-light conditions (10 to 30 μE m–2 s–1, 16 h light). Tall fescue tissues were transformed with three plasmids containing the genes for hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar) and β-glucuronidase (uidA;gus), and red fescue with three plasmids containing hpt, uidA and a synthetic green fluorescent protein gene [sgfp(S65T)]. DNA from T0 plants of eight independently transformed lines from tall fescue and 11 from red fescue were analyzed by PCR and DNA blot hybridization. The co-expression frequency of all three transgenes [hpt/bar/uidA or hpt/uidA/sgfp(S65T)] in transgenic tall fescue and red fescue plants was 25–27%; for two transgenes [hpt/bar or hpt/uidA for tall fescue and hpt/uidA or hpt/sgfp(S65T) for red fescue], the co-expression frequency was 50–75%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000238
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  • 2
    Electronic Resource
    Electronic Resource
    Bialaphos selection of stable transformants from maize cell culture (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 625-631 
    Details
    ISSN: 1432-2242
    Keywords: Microprojectile bombardment ; Bialaphos ; bar gene ; Maize ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stable transformed Black Mexican Sweet (BMS) maize callus was recovered from suspension culture cells bombarded with plasmid DNA that conferred resistance to the herbicide bialaphos. Suspension culture cells were bombarded with a mixture of two plasmids. One plasmid contained a selectable marker gene, bar, which encoded phosphinothricin acetyl transferase (PAT), and the other plasmid encoded a screenable marker for β-glucuronidase (GUS). Bombarded cells were selected on medium containing the herbicide bialaphos, which is cleaved in plant cells to yield phosphinothricin (PPT), an inhibitor of glutamine synthetase. The bialaphos-resistant callus contained the bar gene and expressed PAT as assayed by PPT inactivation. Transformants that expressed high levels of PAT grew more rapidly on increasing concentrations of bialaphos than transformants expressing low levels of PAT. Fifty percent of the bialaphos-resistant transformants tested (8 of 16) expressed the nonselected gene encoding GUS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226875
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    Paper (German National Licenses)
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