ISSN:
1432-203X
Keywords:
Plant cells
;
Genetic modification
;
Stability
;
GUS
;
Tobacco cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independently transformed cell lines either in the presence or the absence of selectable antibiotics. Specific GUS activity was stably maintained in five lines. About a two-to four-fold increase in the GUS activity was observed from three cell lines. The remaining two cell lines lost the activity within the first 70 to 210 days. The presence of antibiotics did not significantly alter the stability of the foreign protein production in all cell lines examined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00234589
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