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  • 1
    Electronic Resource
    Electronic Resource
    gaMS-1: a gametophytic male sterile mutant in maize (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    Details
    ISSN: 1432-2145
    Keywords: Key words Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization of gametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis. gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, non-functional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050034
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 551-555 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Herbicide ; Molecular markers ; Gametophytic selection ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173644
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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