ISSN:
1861-387X
Keywords:
mRNA fingerprinting
;
Polymerase chain reaction
;
Gene expression
;
Glial cell differentiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To identify differentially expressed genes involved in rat C6 glioma cell differentiation induced by cyclic AMP, we adopted mRNA fingerprinting using arbitrarily primed polymerase chain reaction (PCR) (RAP). Four complementary DNA (cDNA) fragments differentially expressed during differentiation were isolated, and they appeared to contain coding regions of corresponding mRNAs. RAP can be used to efficiently identify cDNA fragments by comparing nucleotide and deduced amino acid sequences with those in databases, and is thus a useful method to search for and identify important genes involved in complex cellular processes such as glioma cell differentiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02478880
Permalink