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  • 1
    Electronic Resource
    Electronic Resource
    Mapping resistance to cereal aphids in barley (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 592-596 
    Details
    ISSN: 1432-2242
    Keywords: Key words Barley ; Cereal aphids ; Quantitative trait loci ; Gene mapping ; Insect resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of 150 doubled-haploid (DH) barley (Hordeum vulgare L.) lines derived from the cross of Harrington/TR306 was used to determine the number and chromosomal location of quantitative trait loci (QTLs) controlling resistance to cereal aphids. The experiments were conducted under natural infestation in the field during two growing seasons: 1994 and 1995. Aphid resistance was measured by counting the number of aphids per plot. Counts were made on a weekly basis. Each year at the time of maximum aphid density there was an obvious difference in reaction between the parental genotypes. The DH lines showed continuous variation for aphid density. Simple interval mapping and simplified composite interval mapping revealed that the principal QTL determining cereal aphid resistance is on the distal region of the short arm of chromosome 1. This aphid-resistance QTL is linked with a heading-date QTL. At the time of highest aphid infestation, this QTL accounted for 31% and 22% of the total variance of aphid density in 1994 and 1995, respectively. A QTL on chromosome 5 was also detected but only by simplified composite interval mapping. However, the largest consistent effect was due to the QTL on the short arm of chromosome 1. This QTL may be a useful target for marker-assisted selection for adult plant cereal aphid resistance in barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050455
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    HPLC analysis of human epidermal growth factor using immunoaffinity precolumn. II. Determination of hEGFs in biological fluids (1989)
    Springer
    Chromatographia 27 (1989), S. 574-580 
    Details
    ISSN: 1612-1112
    Keywords: Immunoaffinity chromatography ; Human epidermal growth factor (hEGF) ; Degradation products ; Immunoaffinity precolumn ; HPLC column-switching system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid-chromatographic, column-switching system for automated sample pre-treatment and determination of human epidermal growth factor and its degradation products (hEGFs) is described. The system consists of an immunoaffinity precolumn (4.0×10mm) packed with diol silica immobilized with antibody against hEGF and an analytical ODS column (4.6×250mm). Samples such as cultured media ofE. coli, human urine, milk, seminal fluid and saliva can be directly injected on the immunoaffinity precolumn and the analytes of interest are trapped by the immobilized anti-hEGF antibody. After washing this precolumn with aqueous solvents, the analytes are desorbed with an aqueous solution of low pH and transferred to the analytical column to allow their separation in reversed phase mode. The recoveries of hEGFs spiked in these biological fluids were over 98%. The detection limit was 1 ng for a 1ml sample injection. This method was applied for the determination of hEGF levels in cultured media ofE. coli and biological fluids. Degradation of hEGF in human serum and urine was also examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258981
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    HPLC analysis of human epidermal growth factor using immunoaffinity precolumn. I. Optimization of immunoaffinity column (1989)
    Springer
    Chromatographia 27 (1989), S. 569-573 
    Details
    ISSN: 1612-1112
    Keywords: Immunoaffinity chromatography ; Human epidermal growth factor (hEGF) ; Immobilization of antibody ; Pore size of diol silica ; Optimum coupling conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The preparation of support-coupled antibodies for high-performance immunoaffinity chromatography has been demonstrated. Diol silica had the least non-specific adsorption of all supports investigated. The optimum activation conditions for diol silica with 1,1′-carbonyl-diimidazole and the optimum coupling conditions for anti-human epidermal growth factor antibody as a function of salt concentration, pH and ligand concentration were selected. The pore size of the diol silica was an important factor in achieving good results. Diol silica with 500Å pore size had high loading capacity against the antigen. The use of F(ab′)2 or Fab as a ligand showed almost the same chromatographic characteristics as IgG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258980
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    Paper (German National Licenses)
    Fulltext
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