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  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 97 (1985), S. 737-745 
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Zunehmend beschäftigen sich Peptidchemiker mit der Synthese biologisch und medizinisch wichtiger Polypeptide. In diesem Aufsatz werden einige nützliche Anwendungen von synthetischen Peptiden in Biochemie, Molekularbiologie, Immunologie und Medizin beschrieben. Synthetische Peptide sind von Interesse für Untersuchungen über Struktur-Funktions-Beziehungen bei Polypeptiden, als Peptidhormone und Hormonanaloga, für die Herstellung kreuzreagierender Antikörper und für die Konstruktion neuer Enzyme. Peptidchemiker können zum Fortschritt in den biologischen und medizinischen Wissenschaften beitragen, wenn sie mit deren aktuellen Problemen vertraut sind.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 24 (1985), S. 719-727 
    ISSN: 0570-0833
    Keywords: Peptidomimetics ; Peptides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Peptide chemists are increasingly concerned with the synthesis of polypeptides of biological and medical interest. This article describes several important applications of synthetic peptides in biochemistry, molecular biology, immunology, and medicine. Synthetic peptides may be useful in structure-function studies of polypeptides, as peptide hormones and hormone analogues, in the preparation of cross-reacting antibodies, and in the design of novel enzymes. Peptide chemists can actively contribute to progress in these fields if they are aware of the current problems in the life sciences.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Protein map ; Haemophilus influenzae ; Heparin chromatography ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional gel electrophoresis separates several hundred protein molecules in one single experiment and is efficiently used to study the products expressed by different genomes. Low-copy-number gene products are invisible on a stained two-dimensional map and must be enriched such that sufficient amounts are present for visualization and identification. We investigated the enrichment of proteins of the bacterium Haemophilus influenzae by chromatography on immobilized heparin which has affinity for growth and protein biosynthesis factors. Total soluble proteins of the microorganism were fractionated on Heparin-Actigel which resulted in enrichment of approximately 160 proteins. The eluates, representing about 40% of the applied proteins, were analyzed by two-dimensional gel electrophoresis and the protein spots were characterized by amino acid composition analysis and matrix-assisted laser desorption ionization mass spectrometry. The proteins enriched by chromatography on the heparin gel were not exclusively low-copy-number gene products and they did not exclusively belong to one single class of proteins. The proteins that bound to the heparin gel are indicated in a two-dimensional protein map which includes more than 110 newly identified proteins.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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