ISSN:
1617-4623
Keywords:
Key words Ribosomal proteins S9/L13
;
Genes rpsI/rplM
;
Actinomycetes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The N-terminal amino acid sequences of two peptides derived from a Streptomyces coelicolor ribosomal protein were determined and degenerate oligonucleotide primers derived from these sequences were used as probes for the screening of a chromosomal DNA library of S. coelicolor. Two positive clones were isolated and DNA sequencing of a 1740-bp region of these clones that hybridised with the probes revealed the presence of four genes, two of them incomplete. The deduced products of the two complete genes, rplM and rpsI, showed clear similarities to L13 and S9 ribosomal proteins from various organisms. Promoter-probe and primer extension experiments suggest that the two genes form a single transcriptional unit. The specific rate of synthesis of both proteins was high at early stages of growth but decreased later.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050627
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