ISSN:
1432-0789
Keywords:
Risk assessment
;
Genetically engineered microorganisms
;
Cell-killing function
;
Biological containment
;
Microcosm
;
Suicide mechanism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Data on the use of a biological containment system in microcosms are presented. The system is based on a transposon Tn5-cassette containing a fusion of the lac promoter and gef controlled by lacIQ. The suicide function was inserted into different bacterial hosts and its induction by isopropyl-β-d-thiogalactoside (IPTG) was tested in sterile and non-sterile soil microcosms. The results suggest that the killing effect of this gef-based suicide mechanism is insufficient under soil conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00335852
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