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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a maturation-specific mRNA in dry mung bean embryonic axes (1980)
    Springer
    Planta 149 (1980), S. 227-233 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Immunoprecipitation ; mRNA ; Poly(A) RNA ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poly(A)-rich RNA from dry mung bean (Vigna radiata [L.] Wilczek) embryonic axes has been isolated and translated in a wheat embryo cell-free system, and the products were analyzed on sodium dodecyl sulfate-polyacrylamide gels. The fluorographyic patterns showed a heavy band at approximately MW 12,000. The messenger RNA coding for this polypeptide disappeared in the course of early germination. This messenger is translated in vivo but simultaneously degrades when the axes imbibe. The poly(A)-rich RNA from dry axes has been fractionated on sucrose-dimethyl sulfoxide gradients, and this messenger has been found to be distributed largely in the 9–14 S region. The polypeptide synthesized in vitro has been immunoprecipitated, using the antiserum raised against this protein purified from dry axes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384558
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Trehalose metabolism in dormant and activated spores of Phycomyces blakesleeanus Burgeff (1978)
    Springer
    Planta 139 (1978), S. 171-176 
    Details
    ISSN: 1432-2048
    Keywords: Dormancy ; Phycomyces ; Spores ; Trehalose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is obtained for the existence of two different localizations of trehalase (α,α-trehalose glucohydrolase, EC 3.2.1.28) in Phycomyces spores: one inside the cell, and one in the periplasmic region. The latter enzyme is sensitive to 0.1 mol l-1 HCl treatment and its activity can be regulated by external pH changes. The periplasmic form of the enzyme is involved in the metabolism of added labelled trehalose. This sugar is hydrolyzed externally to glucose which is found mainly in the incubation medium and which is partly absorbed by the spores. During incubation trehalose leaks out from both dormant and activated spores and is subsequently hydrolyzed to glucose. The intracellular trehalase is probably involved in the breakdown of endogenous trehalose in spores. After heat activation the hydrolysis of endogenous trehalose is stimulated even without an important increase in activity of intracellular trehalase. Additional treatments which break dormancy of spores without a significant activation of trehalase are the following: heating of HCl-treated spores and treatment of spores with reducing substances (e.g. Na2S2O4 and NaHSO3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387144
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis and proteolytic activation of chitin synthetase in Phycomyces blakesleeanus Burgeff (1978)
    Springer
    Archives of microbiology 116 (1978), S. 181-184 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Chitin synthetase ; Germination ; Proteolytic activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chitin synthetase of Phycomyces blakesleeanus mycelium is a particulate enzyme sedimenting mostly at 1000xg. The activity in crude extracts or cellular fractions can be increased more than tenfold by mild trypsin treatment. Plotting the reaction velocity versus UDP-N-acetylglucosamine concentration yields a sigmoidal curve. N-acetylglucosamine, which greatly stimulates the enzyme, changes the kinetics to an almost normal hyperbolic relationship. The enzyme is nearly absent in dormant spores and is synthesized “de novo” in germinating spores (from 4 h germination on). Trypsin treatment of extracts from germinating spores to assay the synthesis of the proenzyme did not reveal an earlier synthesis of the zymogen, which therefore might have some activity of its own.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406034
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Density changes of Phycomyces spores after reversible and irreversible activation (1980)
    Springer
    Archives of microbiology 124 (1980), S. 289-291 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Spores ; Germination ; Activation ; Density gradient centrifugation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spores harvested by conventional methods could be cleaned up considerably using discontinuous density gradients. This method yielded a population of almost entirely viable spores. Germination of the spores resulted in density changes probably due to spore swelling. These changes could be observed as early as 15 min after activation. The usefulness of this method in the study of spore activation was shown by its application to spores germinating after a heat treatment at various temperatures, or to spore reacquiring dormancy by incubation in water after activation at different temperatures. Dormant and activated spores can quickly and easily be separated from mixed populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427740
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of gamma radiation on breaking of dormancy in Phycomyces spores (1977)
    Springer
    Archives of microbiology 113 (1977), S. 95-97 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Spore activation ; Dormancy ; Gamma rays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dormant sporangiospores of Phycomyces blakesleeanus were activated by Cs-137 gamma rays. After a dose of about 300 krad, between 80 and 90% of the spores germinated normally. However, further development of the mycelium was inhibited. Even with dry spores a partial activation was obtained. Activation by gamma rays was not accompanied by an increase in trehalase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428587
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Lectin synthesis in developing and germinating wheat and rye embryos (1982)
    Springer
    Planta 156 (1982), S. 41-44 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Lectin ; mRNA (preformed) ; Secale (lectins) ; Seed development ; Triticum (lectins)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (Triticum aestivum L.) and rye (Secale cereale L.) lectins are specifically synthesized during seed formation. They accumulate exponentially in the primary axes in a period coinciding with the development of this complex organ. Since the specific lectin content also increases dramatically, there is apparently an outburst of lectin synthesis during the development of the primary axes. Germinating embryos also synthesize some lectin. The fortunate availability of a highly specific procedure for the isolation of cereal lectins enabled us to follow the kinetics of their synthesis during early germination. Stored mRNAs appear to be involved in this residual lectin synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393441
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    Paper (German National Licenses)
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