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  • 1
    Electronic Resource
    Electronic Resource
    19. Continuous tissue pH measurement on the fetus during delivery (1978)
    Springer
    Archives of gynecology and obstetrics 226 (1978), S. 129-132 
    Details
    ISSN: 1432-0711
    Keywords: pH-Wert-Messung ; Fetaler Säure-Basen-Status ; Gewebe-pH-Wert ; pH-Wert-Elektrode ; pH-Measurement ; Fetal acid-base status ; Tissue-pH ; pH-Electrode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Tissue pH values of 70 fetuses have been measured continuously during labor using the micro-electrode designed by Stamm. Significant correlations between continuous tissue pH and corresponding micro blood values can be shown. However, the relationship between patterns of tissue pH and capillary pH values seems not to be very constant. Therefore, the reliability of individual tissue pH measurements is still questionable.
    Notes: Zusammenfassung Bei 70 Feten wurde während der Geburt mit der von Stamm entwickelten Mikroelektrode der pH-Wert kontinuierlich registriert. Zwischen den Werten der kontinuierlichen pH-Registrierung und den entsprechenden Mikroblutwerten können signifikante Korrelationen aufgezeigt werden. Das Verhalten der beiden Werte (Gewebe-pH/kapilläres pH) ist bei Veränderungen im Säure-Basen-Haushalt jedoch nicht konstant. Deshalb ist die Zuverlässigkeit und die Aussagekraft der kontinuierlichen pH-Messung für den Einzelfall noch fraglich.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02116738
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Assessment of effect of photosensitizers on cytotoxicity of photodynamic therapy in human breast cancer cell cultures (1995)
    Springer
    Archives of gynecology and obstetrics 256 (1995), S. 167-176 
    Details
    ISSN: 1432-0711
    Keywords: Key words: Photodynamic therapy ; Photosensitizers ; m-THPC ; Breast cancer ; In vitro ; Adenosine triphosphate cell viability assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract.  Background: Photodynamic therapy (PDT) might be of clinical value for patients with breast cancer with local recurrences or metastasis. However, there is a need for improved photosensitizers that are effective in combination with laser light and have few, if any, side-effects. We evaluated in vitro the effectiveness of a second generation photosensitizer by testing the influence of laser light on cell cultures of a human breast carcinoma cell line, incubated with meta-tetrahydroxyphenylchlorin (m-THPC) (=Temoporfin®). Experimental design: Five thousand MCF-7 cells were plated in 96-well plates. Forty-eight hours before laser treatment, the cells were plated to achieve a monolayer configuration. Twenty-four hours after plating, they were incubated with m-THPC. On day 6 after treatment with m-THPC we lysed the cells to extract the intracellular ATP that correlates with the number of living cells. The ATP-CVA was used to assess the cytotoxicity of the tested photosensitizer m-THPC at various concentrations and the relevant laser light alone prior to their combination after six days of culture. Results: We found a dose-response for m-THPC alone ranging from 2 to 16 µg/ml. The calculated inhibition concentration to produce 50% cell kill (IC50) was 4.55 µg/ml. We also observed a very low cytotoxicity for laser irradiation alone but a very strong cell kill for the combination of m-THPC together with laser light. Conclusions: PDT gave almost total cell kill at m-THPC concentrations that are not toxic in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00634488
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Assessment of effect of photosensitizers on cytotoxicity of photodynamic therapy in human breast cancer cell cultures (1995)
    Springer
    Archives of gynecology and obstetrics 256 (1995), S. 167-176 
    Details
    ISSN: 1432-0711
    Keywords: Photodynamic therapy ; Photosensitizers ; m-THPC ; Breast cancer ; In vitro ; Adenosine triphosphate cell viability assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Photodynamic therapy (PDT) might be of clinical value for patients with breast cancer with local recurrences or metastasis. However, there is a need for improved photosensitizers that are effective in combination with laser light and have few, if any, side-effects. We evaluated in vitro the effectiveness of a second generation photosensitizer by testing the influence of laser light on cell cultures of a human breast carcinoma cell line, incubated with meta-tetrahydroxy-phenylchlorin (m-THPC) (=Temoporfin®).Experimental design: Five thousand MCF-7 cells were plated in 96-well plates. Forty-eight hours before laser treatment, the cells were plated to achieve a monolayer configuration. Twenty-four hours after plating, they were incubated with m-THPC. On day 6 after treatment with m-THPC we lysed the cells to extract the intracellular ATP that correlates with the number of living cells. The ATP-CVA was used to assess the cytotoxicity of the tested photosensitizer m-THPC at various concentrations and the relevant laser light alone prior to their combination after six days of culture.Results: We found a dose-response for m-THPC alone ranging from 2 to 16 μg/ml. The calculated inhibition concentration to produce 50% cell kill (IC50) was 4.55 μg/ml. We also observed a very low cytotoxicity for laser irradiation alone but a very strong cell kill for the combination of m-THPC together with laser light.Conclusions: PDT gave almost total cell kill at m-THPC concentrations that are not toxic in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00634488
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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