ISSN:
1432-5233
Keywords:
Insulin
;
Glucagon
;
Somatostatin
;
Glucagon-like peptide-I
;
Galanin
;
Incretin
;
Decretin
;
Adenylate cyclase
;
Cyclic adenosine monophosphate
;
Protein kinase A
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The interaction of glucagon-like peptide-I (GLP-I) and galanin in clonal endocrine pancreatic cells was characterized. By Northern blot analysis the presence of GLP-I receptor mRNA was shown in B (\TC-1 cells) and D (RIN 1048-38) cells but not in A (INR1 G9) cells, thus confirming functional data demonstrating the absence of active GLP-I receptors on glucagon-producing cells. Galanin receptors were detected on B and D cells but not on A cells. In B and D cells galanin inhibited the GLP-I stimulated adenylate cyclase activity. Treatment of insulin-and somatostatin-producing cells with GLP-I increased intracellular cAMP levels, and this was dampened by galanin. GLP-I stimulated the activity of protein kinase A in B and D cells, which was also inhibited by galanin. Galanin alone did not influence B- and D-cell function. These data show that in the endocrine pancreas B and D cells but not A cells express GLP-I and galanin receptors. The interaction of GLP-I and galanin occurs at the level of adenylate cyclase. Galanin might act in the endocrine pancreas as a physiological inhibitor of the potent incretin hormone GLP-I. Therefore, we suggest galanin is a ‘decretin’.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00838488
Permalink
