ISSN:
1040-0397
Keywords:
Horseradish peroxidase
;
Glucose oxidase
;
Polypyrrole
;
Glucose
;
Amperometric biosensor
;
Glucose interferences
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Monoenzyme (HRP) and bilyer/monolayer bienzyme (HRP-GOX) bioelectrodes were realized by the oxidative electropolymerization of amphiphilic pyrrole monomer 1 enzyme(s) mixtures, previously adsorbed on the surface of a glassy carbon electrode. Cyclic voltammetry measurements, carried out on poly 1-HRP modified electrodes showed that the electrocatalytical reduction of H2O2, in the presence of K4Fe(CN)6 as mediator, occurs at applied potentials well placed in the optimal potential range for amperometric detection. An optimization study concerning the mediator concentration, the amount of the immobilized enzyme(s), the electrode material and the matrix structure (mono- or bilayer) was performed in order to obtain the glucose biosensor. At an applied potential of -0.1 V (vs. SCE), batch amperometric response to H2O2 for the HRP modified electrodes gave a sensitivity of ca. 280 mA M-1 cm-2 (up to 0.65 mM). For monolayer biosensor the sensitivity to glucose was ca. 170 mA M-1 cm-2 (up to 1.9 mM). The interference of ascorbate, urate and acetaminophen was found almost negligible.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140091306
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