ISSN:
1617-4623
Keywords:
Glutathione S-transferase
;
Maize
;
Gene expression
;
Mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which (Gst I, II andIII) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism including a “null” variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA from “null” and “plus” genotypes, usingGst I- andGst II-specific probes, indicated that theGst I gene controls the expression of the two major GST isoforms expressed in roots.Gst I andGst II were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized.Gst I was localized on the long arm of chromosome 8, while two putativeGst II loci were mapped to chromosomes 8 (70 cM fromGst I) and 10, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02423448
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