ISSN:
1432-0428
Keywords:
Glycation
;
diabetes
;
radioimmunoassay
;
glucitollysine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A radioimmunoassay using antibody against glucitol-lysine was developed to quantitate glycated proteins in the lens of diabetic rats. The amount of glycated protein was expressed as molar equivalents of reduced glycated hippuryl lysine (GlcRED-Hip-Lysine). Significant differences (p〈0.01) were found in the amounts of glycated protein in the lenses of rats with streptozotocin-induced diabetes (3.92±0.59 nmol/mg protein, n=5), those with streptozotocin-induced diabetes treated with insulin (2.94±0.36 nmol/mg protein, n=4) and normal rats (1.23±0.22 nmol/mg protein, n=5). There was a significant correlation between the concentration of glycated protein in the lens and the HbA1c level at the end of the 12 week experiment (r=0.957, p〈0.001). These results indicate that glycation of lens protein is parallel with the severity of diabetes in rats.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290588
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