ISSN:
1432-203X
Keywords:
Grapevine
;
Agrobacterium tumefaciens
;
transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (ß-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segments were subsequently tested in a leaf explant system. On leaves the transformation efficiency of the strains was much lower than with stems. Both the T-6b gene and the hsp 70-T-6b gene (a modified T-6b gene under the control of a heat shock promoter) allowed the initiation of GUS-positive buds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232531
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