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  • Green bean  (1)
  • Keywords Computer-assisted surgery •¶Medical robotics • Orthopedics  (1)
  • 1
    ISSN: 1433-0431
    Keywords: Schlüsselwörter Computerunterstützte Chirurgie •¶Medizinrobotik • Orthopädie ; Keywords Computer-assisted surgery •¶Medical robotics • Orthopedics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract In this paper, we present a medical robot system dedicated to support the surgeon during challenging tasks within orthopedic interventions. The main goal of this work is to develop a system as technically simple as the surgical requirements allow to keep its cost and complexity to a minimum. Therefore, we primarily focus on calibrated X-ray imaging for image acquisition, an easy registration procedure and robotic execution using a positioning device with simple parallel kinematics. Examples of different orthopedic interventions using the compact robot system for image-guided orthopedic surgery (CRIGOS) are presented, as well as various modes of execution of the device.
    Notes: Zusammenfassung Im folgenden Beitrag wird das Konzept und die Entwicklung eines Medizinroboters für die orthopädische Chirurgie behandelt, das den Chirurgen bei der Ausführung komplizierter Eingriffe intraoperativ unterstützten soll. Ein Schwerpunkt der Arbeit ist es, das System so einfach und kostengünstig zu realisieren, wie es die Anwendung erlaubt. Daher greifen wir bei der Bildgebung auf das gängige Verfahren der Röntgenbildgebung zurück. Zur robotischen Ausführung verwenden wir ein einfach, parallel aufgebautes Positioniergerät. Abschließend wird der mögliche operative Ablauf bei Verwendung des bildgeführten Kompaktrobotersystems (CRIGOS) anhand verschiedener orthopädischer Eingriffe exemplarisch aufgezeigt und unterschiedliche Ausführungsstrategien vorgestellt.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Green bean ; Phaseolus vulgaris L ; Genetic transformation ; Stable integration ; chalcone synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin resistant callus was produced from leaf disc or hypocotyl expiants of green bean (Phaseolus vulgaris L.) when cultured on a defined medium containing 50 mg/l kanamycin after 4 days of co-cultivation with Agrobacterium tumefaciens strain EHA101 containing the binary vector pKYLX71GUS. The presence of neomycin phosphotransferase II (NPT-II) in crude cellular extracts from the kanamycin resistant callus was confirmed by ELISA. The expression of the ß-glucuronidase (GUS) reporter gene was confirmed by histochemical and fluorimetric analyses. Southern blot border analysis confirmed the integration of the foreign DNA. In addition to the evidence obtained from Southern analysis, the absence of Agrobacterium in the transformed callus cultures was confirmed by microscopic observation and through test cultures. Using the above protocol, bean callus cultures were also transformed with a bean chalcone synthase promoter-GUS fusion. These cultures, when treated with the elicitor glutathione, showed higher levels of GUS expression than the unelicited callus clumps.
    Type of Medium: Electronic Resource
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