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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 10 (1972), S. 91-102 
    ISSN: 1432-0827
    Keywords: Hydroxyapatite ; Calcification ; Phases ; Growth ; Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La croissance cristalline de l'hydroxyleapatite à 25° et à pH constant de 7.4 a été étudiée à l'aide du microscope électronique à balayage. La technique reproductible de croissance par ensemencement à partir de solutions stables sursaturées est utilisée efficacement pour produire des échantillons de minéral à divers stades distincts de croissance. Des changements de phase sont observés avec le progrès de la croissance; ils correspondent dans le temps avec les résultats cinétiques obtenus antérieurement. Un essai de rationalisation est tenté à la lumière des mécanismes proposés pour la formation d'hydroxyleapatite dans des conditionsin vivo.
    Abstract: Zusammenfassung Das Kristallwachstum von Hydroxyapatit bei 25° und einem konstanten pH von 7,4 wurde mit Hilfe eines Raster-Elektronenmikroskopes studiert. Die reproduzierbare Technik des Keimwachstums aus stabilen übersättigten Lösungen wurde mit Erfolg verwendet, um Mineralproben in verschiedenen bestimmten Stadien des Wachstums zu erhalten. Phasenveränderungen wurden beim fortschreitenden Wachstum beobachtet, und diese stimmten zeitlich gut überein mit kinetischen Resultaten, über welche früher berichtet wurde. Es wurde versucht, diese Beobachtungen zu erklären in Anbetracht von Mechanismen, welche für die Bildung von Hydroxyapatit unterin vivo-Bedingungen vorgeschlagen wurden.
    Notes: Abstract The crystal growth of hydroxyapatite at 25° and at a constant pH of 7.4 has been studied with the aid of a scanning electron microscope. The reproducible technique of seeded growth from stable supersaturated solutions was used effectively to produce samples of the mineral at various distinct stages of growth. Phase changes were observed as the growth proceeded and these corresponded favorably in time with kinetic results reported earlier. An attempt was made to rationalize the observations in light of mechanisms proposed for the formation of hydroxyapatite under conditions foundin vivo.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-0458
    Keywords: Schlüsselwörter Tip-Link-Hypothese ; Lärmschwerhörigkeit ; Schalltrauma ; Tinnitus ; TTS ; Key words Tip link hypothesis ; Noise-induced hearing loss ; Tinnitus ; TTS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Sound overexposure is known to cause damage to cochlear structures and can induce permanent or temporary hearing loss and tinnitus. Perhaps the most sensitive of these structures to sound overexposure are the tip links. In this paper the elecrophysiological effects of pharmacological destruction of the tip links of outer hair cells was investigated. Outer hair cells treated with elastase (20 U/mL) or BAPTA (5 mM) no longer responded to sinusoidal stimuli. In contrast to common belief, transduction channels opened due to loss of tip links. Such opened channels can allow K+ and Ca2+ to enter the cell from the endolymphatic space and could lead to permanent depolarization. This influx of cations caused by loss of tip links, together with the subsequent hair-cell depolarization, might be a source of sensorineural hearing loss and tinnitus associated with acoustic trauma.
    Notes: Zusammenfassung Eine extreme Lärmexposition ist bekannt dafür, Schädigungen des Gehörs hervorzurufen, die sich in permanenter oder temporärer Schwerhörigkeit und Tinnitus manifestieren können. Vor allem die Stereozilien der Haarsinneszellen stellen einen sensiblen Angriffspunkt dar: deren rämliche Ordnung und ihre Verbindungsfilamente (Tip-Links) können zerstört werden. In der vorliegenden Arbeit wurde die Auswirkung einer pharmakologischen Zerstörung der Tip-Links auf das elektrophysiologische Verhalten der Zelle untersucht. Es zeigte sich, dass die behandelten äußeren Haarsinneszellen nicht mehr mechanosensitiv für nasische Stimuli waren, was die Wichtigkeit der Tip-Links bei der Reiztransduktion unterstreicht. Entgegen der allgemeinen Erwartung gingen die mechanoelektrischen Transduktionskanäle aber in einen offenen Zustand über. Durch die permanent offenen Kannäle strömen dauerhaft hauptsächlich K+- und Ca2+-Ionen aus der Endolymphe in die Zelle ein und führen zu einer Depolarisation. Dieser durch den Verlust der Tip-Links verursachte Einstrom von Kationen und die Depolarisation der Zelle können ein Teil der Ursache einer dem Schalltrauma begleitenden Symptomatik wie Innenohrschwerhörigkeit und Tinnitus sein.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1939
    Keywords: Forest decline ; Carbohydrates ; Picea abies ; Growth ; Leaf area index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This is the first in a series of papers on the growth, photosynthetic rate, water and nutrient relations, root distribution and mycorrhizal frequency of two Norway spruce forests at different stages of decline. One of the stands was composed of green trees only while the other included trees ranging in appearance from full green crowns to thin crowns with yellow needles. In this paper we compare the growth and carbohydrate relations of the two stands and examine relationships among growth variables in ten plots. The declining stand produced 65 percent of the wood per ground area compared with the stand in which all trees were green because its foliage produced less wood at any level of leaf area index. The difference in foliage efficiency between the sites could not be explained by differeneces in climate, competition or stand structure. The declining stand appeared to have lower carbon gain as indicated by a smaller increase in reserve carbohydrates before bud break, and weaker sinks for carbohydrates as indicated by less use of the stored carbohydrates than the healthy stand. Thus, growth reduction was probably related to factors which affect both photosynthesis and, even more, the sinks for carbohydrate.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 77 (1988), S. 163-173 
    ISSN: 1432-1939
    Keywords: Forest decline ; Spruce (Picea abies) ; Nutrients ; Growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A declining, closed-canopy Picea abies (L.) Karst. stand produced as much crown biomass as a healthy stand, although some trees were chlorotic due to magnesium deficiency. The production of wood per unit of leaf area in both stands was related to the foliar magnesium concentration. Although leaf area index and climate were similar at both sites, stemwood production was 35% lower in the declining than in the healthy stand. Nutritional disharmony, rather than a deficiency in a single element, was identified as the mechanism for reduced tree vigor. The role of nutrient stress in forest decline was detected by partitioning the season into three periods reflecting different phenological stages: a canopy growth period in spring, a stem growth period in summer, and a recharge period during the non-growing season. Needle growth was associated with nitrogen supply. Most of the magnesium supply required to meet the demand for foliage growth was retranslocated from mature needles. Magnesium retranslocation was related to concentration of nitrogen and magnesium in those needles before bud break. Retranslocation from mature needles during the phase of canopy production resulted in chlorosis in initially green needles if the magnesium concentration before bud break was low. Nitrogen concentration in 0-year-old needles generally remained constant with increasing supply, indicating that foliage growth was restricted by the supply of nitrogen. In contrast, magnesium concentration generally increased with supply, indicating that magnesium supply for needle growth was sufficient. Much of the magnesium required for wood production was taken up from the soil because stored magnesium was largely used for canopy growth. Uptake at the declining site was probably limited because of restricted root expansion and lower soil magnesium compared to the healthy site. For this reason only wood growth was reduced at the declining site. Because the recharge of magnesium during the non-growing period is dependent on uptake from the soil, it was more limited at the declining that at the healthy stand. However, as nitrogen uptake from the atmosphere may account for an appreciable proportion of the total uptake, and as its supply in the soil at both sites was similar, an unbalanced recharge of nitrogen and magnesium may have occurred at the declining site. If mature needles are unable to recharge with magnesium in proportion to the uptake of nitrogen, chlorosis is likely to occur during the next canopy growth period.
    Type of Medium: Electronic Resource
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