ISSN:
1573-5001
Keywords:
Pulsed field gradients
;
HMQC
;
Proteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Three improved versions of the gradient-enhanced HMQC experiment are presented which yield phase-sensitive spectra with increased sensitivity compared to the recently described field-gradient HMQC schemes. The first method uses a complex linear back-prediction in order to generate the FIDs at the t1=0. With this approach, refocusing pulses on the heteronucleus are not necessary. The sequence is especially useful for larger proteins with short relaxation times for the coherences that evolve during t1. In the other two methods lower and shorter gradient pulses or asymmetric gradients are used to optimize sensitivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00178263
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