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  • 1
    Electronic Resource
    Electronic Resource
    Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 458-465 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Barley ; Tissue culture ; Haploid ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plant tissue culture technology is of increasing interest to plant breeders. As part of a continuing investigation into breeding methods with spring barley two studies were conducted to assess the field performance of the progenies of material regenerated in tissue culture. The first study involved two spring barley cultivars, ‘Golden Promise’ and ‘Mazurka’ and compared lines produced from immature embryo (IE) derived callus with those from embryos developed by the Hordeum bulbosum (Hb) technique of chromosome elimination. In general the mean values for the seven characters scored were lower for the IE than the Hb material. In the second study F1 hybrid material (‘Golden Promise’x‘Mazurka’) was used and doubled haploid lines produced by the H. bulbosum and microspore culture (M) techniques were compared with single seed descent (SSD) material. Analysis of these F∝ samples indicated that the mean values for the M lines were significantly lower than those of the Hb and SSD lines. Furthermore, data from the M lines showed significant evidence of variation created during the culture phase. The implications of these findings for barley breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289527
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Anther culture of Hordeum vulgare L.: a genetic study of microspore callus production and differentiation (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 60-64 
    Details
    ISSN: 1432-2242
    Keywords: Barley ; Anther culture ; Pollen ; Haploid ; Callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of the ability of barley anthers to produce microspore-derived callus in vitro was investigated. The genotypes selected were the two spring cultivars ‘Dissa’ (D) and ‘Sabarlis’ (S), the two F1 hybrids (DxS, SxD), the two backcross generations [Dx(DxS), Sx(DxS)], and an F2 generation derived from DxS. From a number of individuals of each generation, the first five spikes were harvested sequentially and after pre-treatment the anthers were removed and placed in culture. Cultures were scored for microspore callus production and plantlet differentiation. Although ‘Dissa’ gave a significantly higher level of callus production than ‘Sabarlis’, the overall frequencies of green and albino plant production were higher from ‘Sabarlis’. There was no significant difference between reciprocal F1 hybrids. Analysis of variance revealed significant differences in response between the spikes sampled from the plants. This was the major source of variation in the experiment. Spike to spike variation also appeared to be a heritable character.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290084
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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