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  • 1
    ISSN: 1432-2242
    Keywords: Zea mays L. ; RFLPs ; Quantitative traits ; Favorable alleles ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Molecular markers can be used to detect alleles in donor genetic material for improvement of existing cultivars or hybrids. DNA restriction fragment length polymorphisms (RFLPs) were used as markers to search for favorable alleles at quantitative trait loci in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid ‘FRB73 x FRMo17.’ Thirty-four RFLP markers were used to determine RFLP ‘fingerprints’ for 220 [BS11(FR)C7 x FRMo17] F2 individuals; multiple morphs (bands) were observed at most markers. Statistical associations between RFLPs and trait expression in F2 x FRB73 progeny were found for grain yield, stalk and root lodging, plant and ear height, maturity, and seven grain yield component traits. Associations were found using linear contrasts among RFLP marker classes to estimate trait effects. Estimated effects for grain yield ranged from 213 to 538 kg ha-1, 3.0–7.5% of the experimental mean, respectively. RFLP markers with greatest probability of association with grain yield were NPI234 (short arm of chromosome 1) and UMC16 (long arm of chromosome 3). Digenic epistasis appeared to be important in grain yield expression, as indicated by a 12% increase in the proportion of genotypic variation accounted for when significant di-marker interactions were added to a linear model, including all markers individually associated with grain yield. The majority of interactions associated with grain yield involved markers NPI234 and UMC21 (long arm of chromosome 6). Many RFLP markers were associated with multiple traits. At some markers, the same bands were associated with favorable effects for stalk lodging, grain yield, and yield components. RFLP bands unique to BS11(FR)C7 showed associations favorable over those from FRMo17 for at least one marker in all but one trait. The results of this study will be useful in future RFLP marker-assisted selection programs aimed at developing lines for improved performance in combination with FRB73.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 87 (1993), S. 217-224 
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Mapping ; Illinois Long Term Selection Experiment ; Quantitative trait loci (QTL) ; Protein ; Starch ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was initiated to determine the number, chromosomal location, and magnitude of effect of QTL (quantitative trait loci or locus depending on context) controlling protein and starch concentration in the maize (Zea mays L.) kernel. Restriction fragment length polymorphism (RFLP) analysis was performed on 100 F3 families derived from a cross of two strains, Illinois High Protein (IHP), X Illinois Low Protein (ILP), which had been divergently selected for protein concentration for 76 generations as part of the Illinois Long Term Selection Experiment. These families were analyzed for kernel protein and starch in replicated field trials during 1990 and 1991. A series of 90 genomic and cDNA clones distributed throughout the maize genome were chosen for their ability to detect RFLP between IHP and ILP. These clones were hybridized with DNA extracted from the 100 F3 families, revealing 100 polymorphic loci. Single factor analysis of variance revealed significant QTL associations of many loci with both protein and starch concentration (P 〈 0.05 level). Twenty-two loci distributed on 10 chromosome arms were significantly associated with protein concentration, 19 loci on 9 chromosome arms were significantly associated with starch concentration. Sixteen of these loci were significant for both protein and starch concentration. Clusters of 3 or more significant loci were detected on chromosome arms 3L, 5S, and 7L for protein concentration, suggesting the presence of QTL with large effects at these locations. A QTL with large additive effects on protein and starch concentration was detected on chromosome arm 3L. RFLP alleles at this QTL were found to be linked with RFLP alleles at the Shrunken-2 (Sh2) locus, a structural gene encoding the major subunit of the starch synthetic enzyme ADP-glucose pyrophosphorylase. A multiple linear regression model consisting of 6 significant RFLP loci on different chromosomes explained over 64 % of the total variation for kernel protein concentration. Similar results were detected for starch concentration. Thus, several chromosomal regions with large effects may be responsible for a significant portion of the changes in kernel protein and starch concentration in the Illinois Long Term Selection Experiment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1619-7089
    Keywords: Adenosine ; Thallium ; Asystole ; Heart ; Sino-atrial disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Adenosine is used increasingly as an alternative to dynamic exercise during myocardial perfusion imaging because it is a powerful coronary vasodilator with a short half-life. Minor side-effects are common but life-threatening events are rare. We report two cases of provocation by adenosine infusion of profound sinus bradycardia progressing to atrial and ventricular asystole. Despite discontinuation of the infusion, asystole persisted for up to 1 min in one case and was accompanied by a grand mal seizure. Normal sinus rhythm returned spontaneously in both cases without long-term sequelae. Sino-atrial disease was later suggested in both cases by 24-h electrocardiographic monitoring. We conclude that patients to whom adenosine is given may have occult sino-atrial disease and may be susceptible to life-threatening arrhythmias. Significant sinus bradycardia during the infusion may provide a warning of its presence.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1615-6692
    Keywords: Key Words Cardiac imaging ; MRI ; Heart ; Ventricular function ; Schlüsselwörter Kardiale Bildgebung ; Magnetresonanztomographie ; Herz ; Ventrikelfunktion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die kardiale Dysfunktion ist eine der Hauptursachen kardiovaskulärer Morbidität und Mortalität. Eine genaue und reproduzierbare Bestimmung der Herzfunktion ist essentiell für die Diagnosestellung, Prognoseabschätzung und Beurteilung des Therapieeffekts beim einzelnen Patienten. Die kardiovaskuläre Magnetresonanztomographie (CMR) bietet eine Messmethode für die globale und regionale Herzfunktion, die nicht nur genau und reproduzierbar, sondern auch nichtinvasiv, ohne ionisierende Strahlung und unabhängig von geometrischen Annahmen und einem akustischen Fenster ist, das den Einsatz der Echokardiographie limitiert. Mit der Verfügbarkeit schnellerer MR-Scanner und automatisierter Analysesysteme sowie mit zunehmender Verbreitung und reduzierten Kosten wird CMR bald den Referenzstandard für die Messung der Herzfunktion darstellen.
    Notes: Abstract Cardiac dysfunction is a major cause of cardiovascular morbidity and mortality. Accurate and reproducible assessment of cardiac function is essential for the diagnosis, the assessment of prognosis and evaluation of a patient's response to therapy. Cardiovascular Magnetic Resonance (CMR) provides a measure of global and regional function that is not only accurate and reproducible but is noninvasive, free of ionising radiation, and independent of the geometric assumptions and acoustic windows that limit echocardiography. With the advent of faster scanners, automated analysis, increasing availability and reducing costs, CMR is fast becoming a clinically tenable reference standard for the measurement of cardiac function.
    Type of Medium: Electronic Resource
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