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  • Heck reaction, intramolecular  (2)
  • Light microscopy  (2)
  • Nucleotide sequence analysis  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Global cerebral ischemia and subsequent selective hypothermia (1991)
    Springer
    Acta neuropathologica 81 (1991), S. 443-449 
    Details
    ISSN: 1432-0533
    Keywords: Global cerebral ischemia ; Post-ischemic selective brain cooling ; Ischemica neuronal damage ; Light microscopy ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new method of external selective brain cooling is described, showing its effectiveness in reducing neuronal damage following global cerebral ischemia in cat. The cooling apparatus consists of a specially fitted kind of water jacket in which the animal's head was laid. In a preliminary study it was verified that the device effectively reduces brain temperature without the risk of cardiac arrhythmias due to lowering of core temperature. In the main study cardiac arrest was induced in 23 adult cats, followed after 15 min by cardiopulmonary resuscitation (CPR). Eight cats could not be revived; of the 15 remaining animals, 7 were assigned to the control group (normothermia) and 8 to the treatment group (cerebral hypothermia). The latter received external brain cooling for 30 min, starting as soon as CPR was begun. Four hours after cardiac arrest all animals were transcardiacally perfused with glutardialdehyde. The brains were stored in fixative and subsequently processed for histopathological and morphometrical evaluation by light microscopy. Analysis of the resulting data showed that animals in the treatment group had a significantly higher percentage of undamaged neurons than animals in the control group, both in the cingulate gyrus (38% vs 10%) and in the parietal cortex (39% vs 14%). The treatment group also had more undamaged neurons in the hippocampus and fewer severely damaged neurons in all three regions, but these differences, though suggestive, were not statistically significant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293466
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and characterization of brnQ, a gene encoding a low-affinity, branched-chain amino acid carrier in Lactobacillus delbrdückii subsp. lactic DSM7290 (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 682-690 
    Details
    ISSN: 1617-4623
    Keywords: Branched-chain amino acid transport ; Lactobacillus delbrückii subsp. lactis ; Nucleotide sequence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene (brnQ), encoding a carrier for branched-chain amino acids in Lactobacillus delbrückii subsp. lactis DSM7290 was cloned in the low-copy-number vector pLG339 by complementation of a transport-deficient Escherichia coli strain. The plasmid carrying the cloned gene restored growth of an E. coli strain mutated in 4 different branched-chain amino acid transport genes at low concentrations of isoleucine, and increased its sensitivity to valine. Transport assays showed that leucine, isoleucine and valine are transported by this carrier and that transport is driven by the proton motive force. Nucleotide sequence analysis revealed an open reading frame of 1338 bp encoding a hydrophobic protein of 446 amino acids with a calculated molecular mass of 47864 Daltons. The start site of brnQ transcription was determined by primer extension analysis using mRNA from Lactobacillus delbrückii subsp. lactis DSM7290. The hydropathy profile suggests the existence of at least 12 hydrophobic domains that probably form membrane-associated α-helices. Comparisons of the nucleotide sequence of brnQ from Lactobacillus delbrückii subsp. lactis DSM7290, the amino acid sequence of its product and the topology of the hydrophobic domains with those of the respective carrier genes and proteins of Salmonella typhimurium and Pseudomonas aeruginosa revealed extensive homology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418038
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and DNA sequence analysis of pepQ, a prolidase gene from Lactobacillus delbrueckii subsp. lactis DSM7290 and partial characterization of its product (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 494-500 
    Details
    ISSN: 1617-4623
    Keywords: Prolidase ; Metalloprotease ; Lactobacillus delbrueckii subsp. lactis ; Nucleotide sequence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From a genomic library of Lactobacillus delbrueckii subsp. lactis (DSM7290) DNA, in the low-copy-number vector pLG339, a recombinant clone was selected, which complemented a mutation in the prolidase gene (pepQ) of Escherichia coli UK173. Nucleotide sequence analysis revealed an open reading frame of 1104 nucleotides corresponding to a protein of 368 amino acids with a calculated pI of 4.64 and a molecular mass of 41087 Da. The start site of pepQ transcription was determined by primer extension analysis with mRNA prepared from L. delbrueckii. Based on homology of the gene product to various peptidases and on the substrate specificity determined, the peptidase was designated PepQ. The influence of various protease inhibitors and cations on peptidase activity indicated that PepQ is a metalloprotease. The absence of a membrane-spanning domain and a signal peptide sequence argues for a cytoplasmic localization of the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293152
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  • 4
    Electronic Resource
    Electronic Resource
    Licht- und rasterelektronenmikroskopische untersuchungen zur pathogenese des sinus pilonidalis und der analristel (1992)
    Springer
    Langenbeck's archives of surgery 377 (1992), S. 118-124 
    Details
    ISSN: 1435-2451
    Keywords: Pilonidal sinus ; Anal fistula ; Light microscopy ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Von den verschiedenen Theorien über die mögliche Entstehung des Sinus pilonidalis ist heute die von Stelzner geäußerte Ansicht eines erworbenen Leidens allgemein anerkannt. Unsere rasterelektronenmikroskopischen Untersuchungen untermauern die Überle-gungen von Stelzner, daß die Haare durch Widerhaken in der Haut festgehalten werden. Der kanülenartige Schliff abgebrochener Haarspitzen mag den Vorgang des Einbohrens in die Haut noch unterstützen. Die Analfistel unterscheidet sich vom Sinus pilonidalis in der Morphologie und der Pathogenese. Die chirurgische Behandlung richtet sich nach der Lage der erkrankten Proktodäaldrii-se.
    Notes: Summary From various theories about the development of the pilonidal sinus Stelzner's opinion as to an acquired disease is generally accepted. Our scanning electron microscopic investigations strengthen this theory that hair splits and drills into the skin. Hooks keep it from retracting. Needle-like sharp ends may contribute to the hair piercing the skin. The anal fistula differs from the pilonidal sinus concerning morphology and pathogenesis. Surgical treatment must consider the localisation of the proctodeal gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184347
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  • 5
    Electronic Resource
    Electronic Resource
    Intramolecular Heck Reaction for the Synthesis of Isochromanes under Ambient and High pressure (1997)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 887-891 
    Details
    ISSN: 0947-3440
    Keywords: Heck reaction, intramolecular ; Isochromane ; Palladium ; Heterocycles ; High-pressure chemistry ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The iodoarenes 9 and 10, bearing (E)- and (Z)-alkene moieties, respectively, easily prepared by alkylation of the corresponding alcohols 7a-c and 8a-c with 2 iodobenzyl iodide, undergo intramolecular Heck reactions to give the isochromanes 12-15a-c in good yields. The selectivity of the reaction depends on the size of the substituent R in 9 and 10; increasing bulkiness of R leads to a decrease in diastereoselectivity and an increase in regioselectivity. High-pressure experiments confirm the proposed mechanism and show that bromoarenes such as 11, which tend to be unreactive at ambient pressure, give good results when the cyclisation is performed under high pressure.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199719970516
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  • 6
    Electronic Resource
    Electronic Resource
    Diastereo- and Regioselective Intramolecular Heck Reaction of α-Amino Alcohol Derivatives for the Synthesis of Enantiomerically Pure Isoquinolines and Benzazepines at Ambient and High Pressure (1997)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 1407-1413 
    Details
    ISSN: 0947-3440
    Keywords: Heck reaction, intramolecular ; α-Amino acids ; Isoquinolines ; Palladium ; Heterocycles ; Synthesis, enantioselective ; High pressure ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Alkylation and acylation of the alkenes 9b, c and 10a, b, which are easily prepared from the corresponding α-amino alcohols, with 2-halobenzyl and 2-halobenzoyl halides respectively, gives 11-15. These compounds cyclize with excellent diastereo- and regioselectivity to the enantiomerically pure N-heterocycles 18-20 in an intramolecular Heck reaction using 5 mol-% of Pd(OAc)2 in the presence of PPh3, TPAB and KOAc. Under the same conditions substrate 17 leads to the enantiomerically pure benzazepine 24. The reaction of the bromoarene derivatives 14-15 must be performed under high pressure to give good results.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199719970718
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