ISSN:
1573-5036
Schlagwort(e):
Glutamine synthetase
;
Helianthus
;
N utilization
;
Nitrate
;
Nitrate reduction
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
Notizen:
Summary It has been reported that in plants of nitrate-fedH. annuus, nitrate reductase activity (NRA) is restricted to the roots of the plant. With an improved extraction technique using a medium containing 2% casein and 1.5 g insoluble polyvinylpyrrolidone (PVP) per gram material, however, the leaves ofH. annuus showed a far greaterin vitro NRA (24.7±0.4 μmoles h−1 g fr.wt.−1) than did the roots (3.4±0.6 μmoles h−1 g fr.wt.−1).In vitro glutamine synthetase activity (GSA) was found to be greater in the leaves (27 μmoles h−1 g fr. wt.−1) than the root (5.6 μmoles h−1 g fr.wt.−1) using a standard extraction medium. With the addition of casein and PVP to the extraction medium, GSA increased to 141 μmoles h−1 g fr.wt.−1 in the leaves and 23 μmoles h−1 g fr.wt.−1 in the roots. It is thus essential to include both casein and PVP in the extracting medium of leaves and roots ofH. annuus when determiningin vitro NRA and GSA.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02182818
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