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  • 1
    Electronic Resource
    Electronic Resource
    Promoter switching during development and the termination site of the σ43 operon of Bacillus subtilis (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 114-119 
    Details
    ISSN: 1617-4623
    Keywords: RNA polymerase ; Promoters ; Bacillus subtilis ; Sigma ; Transcription termination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sequencing data indicated that the RNA polymerase (σ43) operon of Bacillus subtilis consisted of three genes, P23 (function unknown), dnaE (DNA primase), and rpoD (σ43) (Wang and Doi 1986a). S1 nuclease mapping experiments with RNA from various stages of growth demonstrated the presence of two overlapping σ43 promoters that controlled the expression of the operon during growth and a σ37 promoter that regulated the expression of the operon during the sporulation phase. This promoter switching mechanism ensured that this important operon would be expressed during different nutritional states of the cell and also illustrated a function for the minor RNA polymerase σ37 holoenzyme in the expression of genes which are normally expressed during the logarithmic phase of growth. The location of the transcription termination signal confirmed that the σ43 operon consists of three genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331498
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  • 2
    Electronic Resource
    Electronic Resource
    Modulation of oxygen-carrying capacity of artificial red cells (1993)
    New York, NY : Wiley-Blackwell
    Polymers for Advanced Technologies 4 (1993), S. 8-11 
    Details
    ISSN: 1042-7147
    Keywords: Hemoglobin ; Vesicles ; Oxygen affinity ; Allosteric effectors ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The oxygen-carrying capacity of artificial red cells (ARC), which were prepared by encapsulating hemoglobin (Hb) into polymerized lipid vesicles, has been investigated in detail. It was found that the effects of inositol hexaphosphate (IHP) on the oxygen affinity of stromafree Hb and ARC are quite different. By co-encapsulating IHP at a IHP:Hb molar ratio of 1.0, the oxygen affinity of the encapsulated Hb in ARC was decreased to such an extent that P50 was about 60-65 mmHg, and only about 70-80% of the encapsulated Hb could be oxygenated at an oxygen partial pressure (Po2) of about 100 mmHg. As pyridoxal 5-phosphate (PLP) and chloride ions were coencapsulated instead of IHP, P50 and the Hill coefficient of the obtained ARC were adjusted to 25-30 mmHg and 2.5-2.8 respectively. The oxygen-transporting efficiency of PLP-modulated ARC was similar to or better than that of red blood cells.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pat.1993.220040102
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of dehydrated powder of hemoglobin vesicles (1992)
    New York, NY : Wiley-Blackwell
    Polymers for Advanced Technologies 3 (1992), S. 17-21 
    Details
    ISSN: 1042-7147
    Keywords: Hemoglobin ; Vesicles ; Polymerized lipids ; Saccharides ; Freeze-drying ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The carbonyl hemoglobin (CO-Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ-irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze-dried in the presence of saccharides (50-200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long-term storage of Hb vesicles can be realized as a dry powder.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pat.1992.220030103
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    Paper (German National Licenses)
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