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Lippia dulcis shoot cultures as a source of the sweet sesquiterpene hernandulcin (1991)

Sauerwein, Martina ; Flores, Hector E. ; Yamazaki, Takeshi ; [et al.]

Springer

Plant cell reports 9 (1991), S. 663-666

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ISSN: 1432-203X

Keywords: Lippia dulcis ; Shoot culture ; Chitosan ; Hernandulcin

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The axenic shoot culture of Lippia dulcis Trev., Verbenaceae, was established on hormone-free Murashige-Skoog solid medium containing 3% sucrose. Shoots were cultured in various liquid or solid media. Woody Plant liquid medium was best for shoot multiplication, but the production of hernandulcin was relatively low. The highest hernandulcin content (2.9% dry wt) was obtained after 28 days of culture on Murashige-Skoog solid medium containing 2% sucrose. The addition of chitosan to the culture media enhanced the growth of shoots as well as the production of hernandulcin, especially with the liquid medium.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00235352

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The biosynthetic potential of plant roots (1990)

Signs, Mark W. ; Flores, Hector E.

New York, NY : Wiley-Blackwell

BioEssays 12 (1990), S. 7-13

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ISSN: 0265-9247

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: The contribution of roots to the biology of the whole plant is being reevaluated in the light of classical and recent findings. In addition to their role in water and nutrient uptake and in symbiotic associations, plant roots also synthesize a remarkable variety of secondary metabolites. These chemicals, many of which are used as pharmaceuticals, agrichemicals, flavors, dyes, or fragrances, may help the plant cope with biotic and abiotic stress. Root cultures are being used as experimental systems to explore both root-specific secondary metabolites and their biological significance. They may also provide future systems for commercial production of plant specialty chemicals.

Additional Material: 4 Ill.