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  • Heterokaryon incompatibility  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of hch, the Podospora anserina homolog of the het-c heterokaryon incompatibility gene of Neurospora crassa (2000)
    Springer
    Current genetics 38 (2000), S. 39-47 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsPodospora anserina ; Neurospora crassa ; Heterokaryon incompatibility ; het-c
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this gene also functions as a het gene in that species. The het-c and hch sequences are highly similar but differ in the region defining allele specificity in N. crassahet-c. Analysis of hch variability in 11 natural P. anserina isolates with different het genotypes revealed no polymorphism. This suggested that hch does not function as a het gene. However, heterologous expression of the N. crassa het-c PA allele in P. anserina triggers a growth defect reminiscent of the het-c incompatibility reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940000130
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The molecular nature of mutations in the mt A-1 gene of the Neurospora crassa A idiomorph and their relation to mating-type function (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 115-122 
    Details
    ISSN: 1617-4623
    Keywords: Key words Neurospora ; Mating type ; Mutations ; Heterokaryon incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The 293-amino acid mt A-1 ORF of the A mating-type idiomorph of Neurospora crassa is multifunctional. It confers A mating identity and is responsible for heterokaryon incompatibility. The goal of this study was to dissect the functional regions of mt A-1. New mutants of mt A-1 selected for loss of the incompatibility function were obtained. One new mutant, A m99 , was partially fertile as a maternal parent. This is the first time that fertility and incompatibility functions have been separated for the A idiomorph. In this mutant, the mt A-1 ORF is truncated after the first 85 amino acids, indicating that this N-terminal region is minimally sufficient for female fertility. A series of deletion constructs and frameshift alleles of mt A-1 was obtained and tested for male-mating activity and vegetative incompatibility in transformation experiments. These experiments showed that a region from position 1 to 111 is sufficient to confer incompatibility, while amino acids from position 1 to 227 are required for mating activity. A transcriptional analysis of mt A-1 showed that the mRNA is expressed both before and after fertilization. This, together with the phenotype of the A m99 mutant, suggests a post-fertilization function for mt A-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191831
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The molecular nature of mutations in themt A-1 gene of theNeurospora crassa A idiomorph and their relation to mating-type function (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 115-122 
    Details
    ISSN: 1617-4623
    Keywords: Neurospora ; Mating type ; Mutations ; Heterokaryon incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 293-amino acidmt A-1 ORF of theA mating-type idiomorph ofNeurospora crassa is multifunctional. It confersA mating identity and is responsible for heterokaryon incompatibility. The goal of this study was to dissect the functional regions ofmt A-1. New mutants ofmt A-1 selected for loss of the incompatibility function were obtained. One new mutant,A m99 , was partially fertile as a maternal parent. This is the first time that fertility and incompatibility functions have been separated for theA idiomorph. In this mutant, themt A-1 ORF is truncated after the first 85 amino acids, indicating that this N-terminal region is minimally sufficient for female fertility. A series of deletion constructs and frameshift alleles ofmt A-1 was obtained and tested for male-mating activity and vegetative incompatibility in transformation experiments. These experiments showed that a region from position 1 to 111 is sufficient to confer incompatibility, while amino acids from position 1 to 227 are required for mating activity. A transcriptional analysis ofmt A-1 showed that the mRNA is expressed both before and after fertilization. This, together with the phenotype of theA m99 mutant, suggests a post-fertilization function formt A-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191831
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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