ISSN:
1741-0444
Keywords:
Optimal design
;
Receptor concentration
;
PET
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract To estimate in vivo myocardial β-adrenergic receptor concentration with sufficient precision and to reduce the experimental complexities in positron emission tomography (PET), an iterative optimal design method is applied. An initial three-injection protocol, utilising [F-18]-labelled (R)- and (S)-fluorocarazolol and unlabelled (S)-fluorocarazolol, is optimised for ligand dosages and administration times to maximise the precision of all model parameters using the D-optimal criterion. Using this experimental protocol, PET data are collected in porcine studies, and model parameters are estimated. All model parameters are identified with satisfactory precision. The in vivo myocardial β-receptor concentration is 7.5±0.6 pmol ml−1, which corresponds to the in vitro result of 10.1±1.3pmol ml−1. With more accurate parameter values, a simplified two-injection protocol is optimally designed, utilising only radiolabelled and unlabelled (S)-fluorocarazolol, based on a new criterion to maximise the precision of the β-receptor concentration. This revised optimum design predicts that the in vivo β-receptor concentration can be estimated with good precision but reduced experiment complexity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02344863
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