ISSN:
1432-0878
Keywords:
Digestive organs
;
Tissue differentiation
;
Low-Mr-type tropomyosin isoforms
;
High-Mr-type tropomyosin isoforms
;
α and β tropomyosin isoforms
;
Chicken
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Histological localization of tropomyosin isoforms in three digestive organs from embryonic and adult chickens was performed by using rabbit antisera against chicken skeletal muscle tropomyosin and against low-Mr-type tropomyosin from chicken small intestine mucosa. The former antiserum (named TM-SH) reacted with α, β, and high-Mr-type isoforms, and the latter (named TM-HL) reacted with α, β, high-Mr-type and low-Mr-type isoforms. α and β Isoforms were detected in muscle cells of the muscular layer and the muscularis mucosa. Low-Mr-type isoforms, however, were detected along the cell membrane and cytoplasm of almost all nonmuscle cells, especially in terminal webs of epithelial cells. Developmental changes of tropomyosin isoforms in digestive organs were studied by two-dimensional gel electrophoresis and image analysis. The relative amounts of α and β isoforms increased in the course of development, but those of low-Mr-type and high-Mr-type isoforms decreased.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00353894
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