ISSN:
1420-908X
Keywords:
Histamine receptors
;
Histamine
;
Histidine decarboxylase
;
Growth factors
;
Tumor growth
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In order to determine the role of endogenous histamine in the regulation of cell growth, thein vitro action of fluoromethyl-histidine (MFMH) was studied in experimental mammary carcinomas induced in rats. Tumor cells were cultured in soft agar using the clonogenic agar technique. The MFMH was added in different concentrations (0.01–100 μM). The effect observed was a 60% inhibition on colony formation with a maximal effect at concentrations over 10 μM. This action was completely reverted by the H2 agonists dimaprit and arpromidine with an IC50 value of 1 μM. The action of the H2 agonists when added alone was a significant increase in cell proliferation (135%), while the H1 agonist produced a dose-dependent inhibition on cell growth. In these experimental carcinomas endogenous histamine is critical for cell proliferation and one of its major effects may be the stimulation of cell growth by acting on specific H2 membrane receptors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02005757
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