ISSN:
1432-0533
Keywords:
Metachromatic Leukodystrophy
;
Pseudoisocyanine
;
Histochemistry
;
Metachromasia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary With a new staining method, using pseudoisocyanine as a metachromatic dye, accumulations of stored sulfatides in all forms of MLD could be detected on frozen sections of peripheral nerve and other tissues. With pseudoisocyanine sulfatide-micelles develop a “polymeric band” characterized by its maximum absorption at 583 nm (maximum absorption of the monomeric dye is at 530 nm). By staining sulfatide depositions with pseudoisocyanine a red-violet metachromasia is developed on a nearly pale red or colorless background of tissue. The short staining time and the high sensitivity are additional advantages of the method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00687168
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