ISSN:
1573-4935
Keywords:
differentiation
;
Hl-60 cells
;
1α,25 dihydroxyvitamin D3
;
commitment
;
promotion
;
DMSO
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The differentiation of HL-60 cells induced by 1α,25 dihydroxyvitamin D3 was found to be separated into two stages, i.e. commitment and promotion. Most of the HL-60 cells were committed to monocyte/macrophage lineage by pretreatment with 1α,25 dihydroxyvitamin D3 (5–50 ng/ml) for 18–24 hr. The promotion in the second stage was inducer and lineage independent; treatment with 1.25% DMSO for 2 or 3 days promoted the differentiation of the committed HL-60 cells by 1α,25 dihydroxyvitamin D3 into monocyte/macrophage lineage, but not granulocyte lineage.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01145184
Permalink