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  • 1
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum bulbosum ; Fertile triploids ; Gene introgression ; Chromosome substitutions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific hybridisations between Hordeum vulgare L. (cultivated barley) and H. bulbosum L. (bulbous barley grass) have been carried out to transfer desirable traits, such as disease resistance, from the wild species into barley. In this paper we report the results of an extensive backcrossing programme of triploid hybrids (H. vulgare 2x x H. bulbosum 4x) to two cultivars of H. vulgare. Progenies were characterised cytologically and by restriction fragment length polymorphism analysis and comprised (1) haploid and diploid H. vulgare plants, (2) hybrids and aneuploids, (3) single and double monosomic substitutions of H. bulbosum chromosomes into H. vulgare and (4) chromosomal rearrangements and recombinants. Five out of the seven possible single monosomic chromosome substitutions have now been identified amongst backcross progeny and will be valuable for directed gene introgression and genome homoeology studies. The presence amongst progeny of 1 plant with an H. vulgare-H. bulbosum translocated chromosome and one recombinant indicates the value of fertile triploid hybrids for interspecific gene introgression.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum bulbosum ; Powdery mildew resistance ; Gene introgression ; Irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hordeum bulbosum L. is a source of disease resistance genes that would be worthwhile transferring to barley (H. vulgare L.). To achieve this objective, selfed seed from a tetraploid H. vulgare x H. bulbosum hybrid was irradiated. Subsequently, a powdery mildew-resistant selection of barley phenotype (81882/83) was identified among field-grown progeny. Using molecular analyses, we have established that the H. bulbosum DNA containing the powdery mildew resistance gene had been introgressed into 81882/83 and is located on chromosome 2 (2I). Resistant plants have been backcrossed to barley to remove the adverse effects of a linked factor conditioning triploid seed formation, but there remains an association between powdery mildew resistance and non-pathogenic necrotic leaf blotching. The dominant resistance gene is allelic to a gene transferred from H. bulbosum by co-workers in Germany, but non-allelic to all other known powdery mildew resistance genes in barley. We propose Mlhb as a gene symbol for this resistance.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 31 (1982), S. 439-449 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barleygrass ; seed quality ; crossing bag
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of different spike covering materials have been investigated in crosses between Hordeum vulgare and H. bulbosum and in selfed H. vulgare. It was found that after lemmas and paleas were clipped, improvements in seed quality (H. vulgare × H. bulbosum) and weight (selfed H. vulgare) were obtained by covering heads with small brown paper bags compared with other treatments. Possible reasons for these effects are discussed but so far the mechanism has not been clarified. However, light is suggested as playing a major role.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 32 (1983), S. 863-876 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barleygrass ; haploid embryos ; doubled haploids ; genotype influence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between five cultivars of Hordeum vulgare and three genotypes of H. bulbosum and the influence of male and female partners on success rates up to the stage of haploid embryo culture was investigated. Both parents markedly affected seed setting and rates of embryo differentiation, whereas seed quality was mainly influenced by the female. There was an interaction between certain genotypes when overall embryo culture rates were analysed. Following embryo culture only the influence of the female partner was assessed but differences were found between the H. vulgare cultivars regarding rates of hybrid (VB) production and chromosome doubling whereas total plant regeneration was unaffected.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 29 (1980), S. 369-377 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; interspecific cross ; incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Partial incompatibility has already been reported between Hordeum vulgare and H. bulbosum during the course of doubled haploid production, and in this paper attempts to overcome the breeding barrier are described. The methods which seem to offer most success are those of environmental adjustments and the adoption of new genotypes of H. bulbosum. Further cultivars of H. vulgare exhibiting this phenomenon are also noted.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; embryo culture ; haploids ; hybrids ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant regeneration rates from embryos derived from Hordeum vulgare x H. bulbosum were recorded over a 3 1/2 year period. % total plant regeneration (haploids + VB hybrids) varied to some extent but did not seem to be influenced by season or male parent. % VB production (and thus chromosome elimination) was however markedly affected by season and the genotype of the pollinator.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; haploid embryos ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Improvements in the success of doubled haploid production have been achieved partly by screening new accessions of Hordeum bulbosum. In order to assess levels of variation within stocks of this species, rates of embryo differentiation from the cross between H. vulgare × H. bulbosum were recorded for selections derived from two different stocks of H. bulbosum. There was little difference within stocks for this character despite variation in the morphology and banding patterns of two enzyme systems of one of the stocks. It is proposed that to obtain further increases in success rates a few selections from many accessions of H. bulbosum should be screened rather than many selections from a few stocks.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; doubled haploids ; genetype evaluation ; interspecific hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight Hordeum bulbosum selections were produced from a cross between Cb 2920/4 and Cb 2929/1, two genotypes widely used in doubled haploid breeding programmes. The selections were hybridized with barley to evaluate their ability to produce high proportions of well-differentiated haploid embryos compared with Cb 2929/1 as control. We report here an initial small-scale investigation followed by a larger-scale test in two different environments to assess seed setting, haploid embryo differentiation rates and VB hybrid formation. These VB embryos contain both parental sets of chromosomes and occur more frequently in the glasshouse during the winter. Two of the eight selections were identified as combining the desirable characteristics of both parents, namely high seed setting on cv. Vada which is partially incompatible with H. bulbosum, large numbers of well-differentiated haploid embryos and a low incidence of VB hybrids. The selections are available for release to interested research workers and plant breeders
    Type of Medium: Electronic Resource
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