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  • Hordeum vulgare L.  (1)
  • Inverted repeat  (1)
  • Key words Hordeum vulgare  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Short inverted repeats function as hotspots of intermolecular recombination giving rise to oligomers of deleted plastid DNAs (ptDNAs) (1997)
    Springer
    Current genetics 31 (1997), S. 179-184 
    Details
    ISSN: 1432-0983
    Keywords: Key words Intermolecular recombination ; Inverted repeat ; Oryza sativa ; Plastid DNA (PtDNA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have determined the nucleotide sequences around the junction points of oligomeric-deleted ptDNAs possessing a head-to-head or tail-to-tail configuration from long-term cultured cell lines and albino plants. It was shown that DNA rearrangement occurred by direct fusion of deleted ptDNAs in an inverted orientation, which was linked by an asymmetrical sequence of 254–698 bp derived from either of the ptDNAs joined. It is notable that inverted repeats of 7–14 bp flank the asymmetrical sequences at each of the junction points. These features of the DNA sequence around the junction points are commonly observed in oligomeric ptDNA with a large-scale deletion regardless of the cell lines employed. It is suggested that the short inverted repeats are involved in the intermolecular recombination of ptDNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050193
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.) (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 937-946 
    Details
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare ; Linkage map ; Recombinant inbred ; Sequence-tagged site (STS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to identify sequence-tagged sites (STSs) appropriate for recombinant inbred lines (RILs) of barley cultivars ‘Azumamugi’ × ‘Kanto Nakate Gold’, a total of 43 STS primer pairs were generated on the basis of the terminal sequences of barley restriction fragment length polymorphism (RFLP) clones. Forty one of the 43 primer pairs amplified PCR products in Azumamugi, Kanto Nakate Gold, or both. Of these, two showed a length polymorphism and two showed the presence or absence of polymorphism between the parents. PCR products of the remaining 37 primers were digested with 46 restriction endonucleases, and polymorphisms were detected for 15 primers. A 383.6-cM linkage map of RILs of Azumamugi×Kanto Nakate Gold was constructed from the 19 polymorphic STS primer pairs (20 loci) developed in this study, 45 previously developed STS primer pairs (47 loci), and two morphological loci. Linkage analysis and analysis of wheat-barley chromosome addition lines showed that with three exceptions, the chromosome locations of the STS markers were identical with those of the RFLP markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051153
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of random amplified polymorphic DNA (RAPD) markers linked to the v locus in barley, Hordeum vulgare L. (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 637-642 
    Details
    ISSN: 1432-2242
    Keywords: Key words Molecular marker ; v locus (kernel row type) ; Hordeum vulgare L. ; RAPD ; Recombinant backcross line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Recombinant backcross lines of barley were produced from a cross between Kanto Nakate Gold (KNG; two-rowed) and Azumamugi (AZ; six-rowed) after backcrosses of F1 plants with AZ as the recurrent parent. Each of these lines had an introgressed segment from chromosome 2 of KNG. Two recombinant backcross lines, L1 and M3-13, were used for an initial screening of polymorphism. After screening a total of 888 oligonucleotides as arbitrary primers, we identified eight random amplified polymorphic DNAs (RAPDs) between backcross lines and AZ. Among the RAPD fragments, CMNA-38700 was linked to the v locus with a recombination frequency of zero, while OPJ-09850 and OPP-02700 were linked to the v locus at a map distance of 1.4 cM. Thus, the three RAPD markers were clustered around the v locus since the lengths of introgressed chromosomal segments in the L1 and M3-13 lines were no less than 38 cM. The other five RAPD fragments that we identified were not linked to the v locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050606
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    Paper (German National Licenses)
    Fulltext
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