ISSN:
0263-6484
Keywords:
Human bone cell cultures
;
calcitonin action
;
45Ca incorporation
;
cyclic nucleotides
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: (1) a transitory increase in 45Ca uptake that returned to control values ten minutes after the hormone was added; (2) a relation between 45Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; (3) a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db-cAMP or db-cGMP and (4) inhibition of 45Ca uptake and parallel decrease in cAMP levels with propanol.These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45Ca uptake, probably by activating an adenylate-cyclase system through a β-receptor.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cbf.290030304
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