ISSN:
1432-5233
Keywords:
Diabetes-prone BB rats
;
Displacement
;
Human diabetic sera
;
Monoclonal islet cell reactive autoantibodies
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Two fusion experiments using the heteromyeloma cell line CB-F7 and splenocytes from two diabetesprone BB (BioBreeding) rats at the onset of diabetes resulted in 128 islet cell reactive autoantibodies primarily detected with permeabilized insulin-producing rat insulinoma cells (RIN) by a cellular enzyme-linked immunosorbent assay. Seventy-nine (62%) of 128 RIN cell reactive supernatants exhibited a cross-reactivity with rat splenic lymphocytes. Six stable hybridomas secreting monoclonal ICSA (islet cell surface antibodies) were established, but only one monoclonal antibody, R4B10, showed preferential beta-cell binding. Six monoclonal antibodies showed a dual reactivity as ICA (islet cell cytoplasmic antibodies) detected by immunostaining of pancreatic islet cryosections and as ICSA on the surface of viable islet cells, whereas two reacted only with an ICA-like pattern. One monoclonal ICSA was specifically displaced from the RIN cell surface by sera of type 1 diabetic patients.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00569930
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