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  • Human impact  (1)
  • py-1  (1)
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  • 1
    ISSN: 1432-2242
    Keywords: Key words CAP ; py-1 ; RAPD ; RFLP ; Breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report the molecular mapping of the py-1 gene for resistance to corky root rot [Pyrenochaeta lycopersici (Schneider and Gerlach)] in tomato using RAPD and RFLP marker analysis. DNA from near-isogenic lines (NILs) of tomato differing in corky root rot resistance was screened with 575 random oligonucleotide primers to detect polymorphic DNAs linked to py-1. Three primers (OPW-04, OPC-02, OPG-19) revealed polymorphisms between the NILs. Twelve resistant and eight susceptible DNA pools derived from segregating F3 families were used to confirm that the RAPD markers were linked to the py-1 gene. Two of the linked amplified fragments, corresponding to OPW-04 and OPC-02, were subsequently cloned and mapped on the tomato molecular linkage map as RFLPs. These clones were located between TG40 and CT31 on the short arm of chromosome 3. Further analysis with selected RFLP markers showed that 7% (8.8 cM) of chromosome 3 of the resistant line ‘Moboglan’ was introgressed from the L. peruvianum donor parent. Three RFLP markers (TG40, TG324, and TG479) from the introgressed part of chromosome 3 were converted to cleaved amplified polymorphism (CAP) markers for use in a polymerase chain reaction (PCR) assay. These PCR markers will allow rapid large-scale screening of tomato populations for corky root rot resistance.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-6278
    Keywords: Human impact ; Southeastern Australia ; Late Holocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Archaeology , Biology
    Notes: Abstract There are few historical analyses quantifying impacts of human activity in Australia. This paper compares vegetation change, fire regime, erosion and eutrophication rates between the European period and the recent prehistoric past in two lake systems on the south coast of New South Wales. The variance in pollen abundance and hence species population changes increased markedly in the historical period, especially amongst understorey taxa, and this could be related to changes in the local fire regimes and to the effects of grazing. Local fire activity decreased from the prehistorical period at both sites. Erosion rates increased in the historical period and both organic and inorganic components were deposited in the lakes. Erosion episodes could be related to fire during some periods but are clearly controlled by forest disturbance and land-use at other periods. The trophic status of the lakes was increasing from before European settlement but accelerated in the recent past. This was in part due to the increased erosion rates and in part due to fertiliser application. The results suggest that lower rates of erosional and eutrophic change occur in catchments with basaltic than with Holocene sand substrata.
    Type of Medium: Electronic Resource
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