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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    European journal of epidemiology 8 (1992), S. 198-205 
    ISSN: 1573-7284
    Schlagwort(e): Human intestinal spirochetes ; DNA cloning ; Non-radioactive probe ; Restriction site polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract HindIII cleaved human intestinal spirochete genomic DNA was cloned into the plasmid vector pBluescript. The inserts from three of these clones (pRB.C33, pRB.C47 and pRB.F1I) successfully hybridized with DNA from the nine human intestinal spirochetes used in this study. The fourth insert (from clone pRB.A8) failed to hybridize with DNA from two of these isolates (HRM4 and HRM9), and, all four of the inserts hybridized with the Ml strain of swine intestinal spirochete Treponema innocens. Hybridization with DNA from the P18 strain of Treponema hyodysenteriae was observed only for the probe pRB.FII. No hybridization was detected with the human strain of Borrelia burgdorferi. With these probes we were thus able to demonstrate, in southern blot experiments, a restriction site polymorphism among the isolates of the human intestinal spirochetes we tested.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    European journal of epidemiology 3 (1987), S. 187-195 
    ISSN: 1573-7284
    Schlagwort(e): Human intestinal spirochetes ; Swine intestinal treponemes ; T. hyodysenteriae ; T. innocens ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The ultrastructure of twenty human intestinal spirochetes was analyzed using the electron microscope. Negatively stained cells were generally found to be loosely and irregularly waved. The isolates had cell dimensions ranging from 0.12–0.35 μm in width and from 3.9–14.2 μm in length. Twin bundles of flagella were present in the space between the cytoplasmic membrane and the outer membrane. The majority of isolates had five flagella inserted sub-terminally at each cell end. Human intestinal spirochetes divide by binary fission. They are morphologically similar to swine intestinal treponemes, both pathogenic (Treponema hyodysenteriae) and non pathogenic (Treponema innocens), and different from Treponema pallidum, Treponema phagedenis and Borrelia burgdorferi. Following treatment with sodium deoxycolate, no bundles of cytoplasmic microtubules were observed in cells obtained from cultures of human and swine intestinal spirochetes or from cells of B. burgdorferi, while these structures were present in similarly treated cells of T. pallidum and T. phagedenis.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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