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  • 1
    Electronic Resource
    Electronic Resource
    Light requirements for regeneration of protoplasts of the moss Physcomitrella patens (1983)
    Springer
    Planta 157 (1983), S. 39-45 
    Details
    ISSN: 1432-2048
    Keywords: Bryophyta ; Light and regeneration ; Moss protoplast ; Physcomitrella ; Polarity (cell regeneration) ; Protoplast regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts prepared by enzymic treatment of protonemata of the moss Physcomitrella patens regenerate rapidly in white light (15 W m−2). The great majority of protoplasts follow a simple regenerative sequence, namely: cell wall synthesis; formation of an asymmetric cell; division of the asymmetric cell, and further extension and division to produce a new chloronemal filament. Only cell wall formation occurs independently of light. The production of an asymmetric cell requires relatively high photon fluence rates of blue or red light and ceases upon transfer to darkness. The subsequent stages of regeneration require much lower photon fluence rates, and red light is considerably more effective than blue or far-red light in permitting cell division. This system is of interest in the study of the induction of cell polarity in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394538
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosome pairing in a Lolium temulentum X Lolium perenne diploid hybrid with a low chiasma frequency (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 516-522 
    Details
    ISSN: 1432-2242
    Keywords: Lolium ; Hybrid ; Synaptonemal complex ; Crossing over ; Meiotic pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Despite an average difference of about 50% in DNA amount, homoeologous chromosomes pair effectively at first metaphase in the diploid interspecific hybrid between Lolium temulentum and Lolium perenne. However, in the presence of accessory B chromosomes and “diploidising genes” pairing at metaphase I is severely reduced. Reconstruction of serial electron micrographs through pollen mother cell nuclei show that synaptonemal complexes are formed at pachytene between not only homoeologous but also non-homologous chromosome segments resulting in multivalent formation. These associations are largely ineffective in terms of chiasma formation and degenerate at late pachytene. It is highly probable that the pairing determinants exercise their control on chromosome pairing largely by prohibiting the siting of crossovers in homoeologously paired chromosome segments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289188
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    Paper (German National Licenses)
    Fulltext
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