ISSN:
1432-0983
Keywords:
Hygromycin
;
Phleomycin
;
GUS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The barley leaf scald fungus,Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using thehph gene fromE. coli and theble gene fromStreptoalloteichus hindustanus under the control ofAspergillus nidulans promoter and terminator sequences. Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 μg of DNA and 5x107 protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under nonselective, conditions for several generations. Co-transformation using theE. coli uidA gene under the control ofA. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02426964
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