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  • 1
    Electronic Resource
    Electronic Resource
    Monensin and gramicidin stimulate CH4 formation from H2 and CO2 in Methanobacterium thermoautotrophicum at low external Na+ concentration (1986)
    Springer
    Archives of microbiology 146 (1986), S. 181-185 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Na+ dependent methanogenesis ; Na+/H+ antiporter ; Monensin ; Gramicidin ; Uncoupler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methane formation from H2 and CO2 in methanogenic bacteria is a Na+-dependent process. In this communication the effects of Na+ ionophores, of uncouplers, and of Na+/H+ antiporter inhibitors on methane formation from H2 and CO2 were studied with Methanobacterium thermoautotrophicum. 1. Na+ ionophores (the Na+/H+ antiporters monensin and lasalocid and the Na+ uniporter gramicidin) stimulated methanogenesis at lwo external Na+ concentrations when the K+ concentration was high. The ionophores had no effect at high external Na+ concentrations and were inhibitory at low external K+ concentrations. 2. Uncouplers (protonophores and valinomycin plus K+) inhibited methanogenesis at low external Na+ concentration at both low and high external K+ concentrations. Inhibition by uncouplers was relieved by the addition of either Na+ or Na+ ionophores. 3. Na+/H+ antiporter inhibitors (harmaline, amiloride, and NH 4 + ) inhibited methanogenesis at low external Na+ concentration. Inhibition was relieved by the addition of either Na+ or of the Na+ ionophores. The results are discussed with respect to the role of Na transport across the cytoplasmic membrane in methanogenesis from H2 and CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402348
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  • 2
    Electronic Resource
    Electronic Resource
    Testing the “methanochondrion concept”: are nucleotides transported across internal membranes in Methanobacterium thermoautotrophicum? (1987)
    Springer
    Archives of microbiology 146 (1987), S. 370-376 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Nucleotide transport ; Nucleotide binding ; Protoplasts ; Membrane vesicles ; Methanochondrion concept
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to test the “Methanochondrion concept”, uptake of adenine nucleotides in various membrane preparations of Methanobacterium thermoautotrophicum was studied. The uptake showed properties which are in general interpreted as indicative of a transport mechanism: (i) kinetics in the time range of minutes, (ii) temperature dependence, (iii) substrate specificity and (iv) failure to remove the substrate by extensive washing. However, nucleotide transport as an interpretation of this “uptake” can definitely be excluded. Not only an exchange mechanism of the mitochondrial type, but also a general exchange or an uniport mechanism was ruled out. In contrast, the “nucleotide uptake” was shown to be actually a tight and specific binding of ADP and ATP to binding sites at the interior side of the cell membrane. This was conclusively demonstrated in protoplasts obtained from M. thermoautotrophicum cells. In these protoplasts which do not contain internal membranes also nucleotide binding was observed, but only after disruption of the plasma membrane by osmotic lysis, which leads to the exposure of binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410938
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 190-194 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415283
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  • 4
    Electronic Resource
    Electronic Resource
    Oxidation of organic compounds to CO2 with sulfur or thiosulfate as electron acceptor in the anaerobic hyperthermophilic archaea Thermoproteus tenax and Pyrobaculum islandicum proceeds via the citric acid cycle (1994)
    Springer
    Archives of microbiology 162 (1994), S. 286-294 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Thermoproteus tenax ; Pyrobaculum ; islandicum ; Hyperthermophiles ; Archaea ; Acetyl-CoA oxidation ; Citric acid cycle ; Sulfur respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The oxidation of organic compounds with elemental sulfur or thiosulfate as electron acceptor was studied in the anaerobic hyperthermophilic archaea Thermoproteus tenax and Pyrobaculum islandicum. T. tenax was grown on either glucose or casamino acids and sulfur; P. islandicum on peptone and either elemental sulfur or thiosulfate as electron acceptor. During exponential growth only CO 2 and H2S rather than acetate, alanine, lactate, and succinate were detected as fermentation products of both organisms; the ratio of CO2/H2S formed was 1 : 2 with elemental sulfur and 1 : 1 with thiosulfate as electron acceptor. Cell extracts of T. tenax and P. islandicum contained all enzymes of the citric acid cycle in catabolic activities: citrate synthase, aconitase, isocitrate dehydrogenase (NADP+ -reducing), oxoglutarate : benzylviologen oxidoreductase, succinyl-CoA synthetase, succinate dehydrogenase, fumarase and malate dehydrogenase (NAD+-reducing). Carbon monoxide dehydrogenase activity was not detected. We conclude that in T. tenax and P. islandicum organic compounds are completely oxidized to CO2 with sulfur or thiosulfate as electron acceptor and that acetyl-CoA oxidation to CO2 proceeds via the citric acid cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301853
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    Paper (German National Licenses)
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