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  • 1
    Electronic Resource
    Electronic Resource
    Silver staining of the neuronal nucleus in electron microscopy: Nucleolar organization in supraoptic nuclei of the rat hypothalamus (1981)
    Springer
    Anatomy and embryology 163 (1981), S. 265-273 
    Details
    ISSN: 1432-0568
    Keywords: Silver staining ; Neurons ; Nucleolus ; Supraoptic nucleus ; Hypothalamus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present paper describes a simple, efficient method for silver impregnation of supraoptic nuclei of the rat hypothalamus using a modification of the ammoniacal silver technique of Cajal (1903). This procedure, involving a silver-developer sequence in tissue blocks prior to plastic embedding, permits the simultaneous study of Ag-impregnated supraoptic neurons at both light and electron microscopic levels. Visualization of secretory magnocellular neurons impregnated by this technique using the electron microscope reveals a good preservation of nuclear structures. A selective accumulation of silver grains was observed over heterochromatin clumps and nucleoli, which allows the identification of the nucleolar fibrillar centers and also the dense fibrillar component as the main areas involved in the silver reaction. The meaning of such a silver-distribution pattern is discussed in the light of recent ultrastructural and biochemical data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315704
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Nuclear inclusions in immature glial cells of the rat hypothalamus during the postnatal period (1983)
    Springer
    Anatomy and embryology 167 (1983), S. 263-271 
    Details
    ISSN: 1432-0568
    Keywords: Nuclear inclusions ; Immature glial cells ; Hypothalamus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural study of the hypothalamic ventromedial nucleus during the postnatal period showed the presence of nuclear inclusions in immature glial cells. These inclusions, identified as “coiled bodies”, consist of round-to-oval formations of coiled electron-dense strands embedded in a less dense fibrillar matrix. Coiled bodies are located free within the nucleoplasm, showing no specific relationships with the nucleolus or the nuclear membrane. The cells containing coiled bodies were typified as oligodendrocyte precursors, mainly oligodendroblasts. The coiled bodies were not found in mature glial cells nor in other types of immature glial elements. The nature and possible functional role of coiled bodies are suggested in the light of recent morphological and biochemical data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298515
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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