ISSN:
1612-1112
Keywords:
Column void time
;
Reversed-phase HPLC
;
Vacancy peak
;
IR direct monitoring
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The elution characteristics of some solutes commonly used to measure column void time on three reversedphase stationary phases in micro-HPLC are described. The elution characteristics of sodium nitrie, sodium nitrate, deuterium oxide, acetone and N,N-dimethylformamide were determined by using styrene-divinylbenzene copolymer, octylsilica and octadecylsilica columns with aqueous methanol and/or acetonitrile eluents. The results indicate that sodium nitrite and sodium nitrate are suitable solutes in measuring column void time, and that the elution behavior of deuterium oxide is almost similar to that of both sodium salts but it appears as two peaks on a C-18 column. One of the two peaks is confirmed as “vacancy” by the use of the micro-HPLC/IR direct monitoring system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02270671
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