ISSN:
1573-0603
Keywords:
B lymphocytes
;
Epstein-Barr virus
;
Immortalization
;
Lymphoblastoid cell line
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00986653
Permalink
