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  • 1
    Electronic Resource
    Electronic Resource
    Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus (1995)
    Springer
    Methods in cell science 17 (1995), S. 67-74 
    Details
    ISSN: 1573-0603
    Keywords: B lymphocytes ; Epstein-Barr virus ; Immortalization ; Lymphoblastoid cell line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00986653
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