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  • Immunoelectron microscopy  (1)
  • Key words Mammalian-type thyrotropin  (1)
  • Squamous cell carcinomas  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Epidermal growth factor receptor expression on oral mucosa dysplastic epithelia and squamous cell carcinomas (1992)
    Springer
    European archives of oto-rhino-laryngology and head & neck 249 (1992), S. 243-247 
    Details
    ISSN: 1434-4726
    Keywords: Epidermal growth factor receptor expression ; Normal oral mucosa ; Dysplastic epithelia ; Squamous cell carcinomas ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of the receptor for epidermal growth factor (EGF) has been determined on oral squamous cell carcinomas. Immunoreactive receptor was localized using a monoclonal anti-EGF-receptor antibody which reacts with sequences in the external domain of the receptor. Frozen sections were studied from 40 patients with squamous cell carcinomas. In 16 sections from the patients with the squamous cell carcinomas, normal differentiated oral mucosa was included and in 7 of these the patients had received preoperative radiotherapy. Sections from 6 other patients with squamous cell carcinoma contained dysplastic epithelia. EGF-receptor-positive cells were present in the basal cell layer on normal differentiated oral mucosa. In sections from patients receiving preoperative radiotherapy the EGF-receptor-positive cells were also found in the spinous cells. In dysplastic epithelia nearly all cells stained for the receptor. The distribution and staining intensity of the EGF receptor varied in the oral squamous cell carcinomas, 36 were positive. The staining pattern in the carcinomas obtained from patients receiving preoperative radiotherapy was not altered qualitatively. Nearly all poorly differentiated cells were stained, but when the tumor was moderately to well differentiated a reduction in the extent of staining in certain areas was seen, paralleling the findings observed in the differentiated upper layers of the normal oral mucosa. This was most pronounced for the epithelial pearls, where the EGF-receptor-positive cells were localized to the undifferentiated cells in the periphery. The results of the present investigation confirm the presence of the EGF receptor on undifferentiated cells, with the extent of the staining reaction on oral squamous cell carcinomas varying inversely with cellular differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00714485
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural immunocytochemical localization of vitellogenin in the fat body of the blowfly, Calliphora vicina Rob.-Desv. (erythrocephala Meig.) by use of the unlabeled antibody-enzyme method (1980)
    Springer
    Cell & tissue research 208 (1980), S. 445-455 
    Details
    ISSN: 1432-0878
    Keywords: Calliphora ; Fat body ; Vitellogenin ; Immunoelectron microscopy ; Hormone regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The unlabeled antibody-enzyme method was used to demonstrate ultrastructurally the specific localization of vitellogenin in the fat body of Calliphora. In control flies the binding sites to vitellogenin were localized in secretory granules situated in the Golgi complex, and in larger bodies named composite secretory granules. These composite granules appear to be formed when a part of a Golgi complex containing secretory granules and a number of small vesicles become surrounded by a common membrane. Ovariectomized flies, which apparently do not produce secretory granules, exhibited no immunocytochemical staining. Ovariectomized flies in which the administration of ecdysterone induced formation of secretory granules, also revealed specific staining on these granules. This is the first ultrastructural evidence of: (a) the specific localization of vitellogenin in secretory granules of the fat body of an insect; (b) the relationship between the presence of the ovary, and of ecdysterone, and the synthesis of vitellogenin by the fat body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233876
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization and characterization of mammalian thyrotropin-like material in the pituitary of the Australian lungfish, Neoceratodus forsteri (1998)
    Springer
    Cell & tissue research 294 (1998), S. 515-523 
    Details
    ISSN: 1432-0878
    Keywords: Key words Mammalian-type thyrotropin ; Pituitary ; Immunocytochemistry ; Australian lungfish ; Neoceratodus forsteri (Dipnoi)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The binding sites of polyclonal antisera raised against the β-subunit of human thyroid-stimulating hormone (hTSHβ), hTSH, and ovine TSH (oTSH) have been localized in the pituitary gland of the Australian lungfish, Neoceratodus forsteri, using light microscopy. Reactivity toward anti-TSH antiserum was demonstrated in a slightly elongated and irregularly-shaped distinct cell type forming clusters in the dorso-central and ventral regions of the distal lobe. Their granules react with alcian blue (AB), and with periodic acid–Schiff (PAS), and after AB-PAS-orange G they stain blue or purple. The specificity of the different antisera was established by liquid-phase absorptions and confirmed in positive and negative tissue control systems. Our observations confirm that dipnoan (Neoceratodus) TSH shares a number of antigenic determinants with those of mammalian TSHβ and support the concept that mammalian TSHβ, or part of it, was established early in evolution, and that dipnoans (Neoceratodus) as living sarcopterygians may have an ancestor in common with the early amphibians. The mapping and detailed description of TSH-like immunoreactive cells may furnish a background to facilitate current and future analysis of the ontogeny and time course of TSH production and release in Neoceratodus in relation to different physiological conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051202
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    Paper (German National Licenses)
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