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  • Monoclonal antibodies  (2)
  • Immunogold labelling  (1)
  • Ontogeny  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immuno-architecture of human fetal lymphoid tissues (1988)
    Springer
    Virchows Archiv 413 (1988), S. 563-571 
    Details
    ISSN: 1432-2307
    Keywords: Fetal lymphoid tissue ; Immunohistology ; Monoclonal antibodies ; Ontogeny ; Paraffin embedded tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Spleen, thymus and lymph node of human fetuses from the 12th to the 38rd week (spleen from 9 weeks) were investigated in an immunohistological study on B5-fixed paraffin embedded tissues, employing a panel of recently developed monoclonal antibodies, reactive with antigens resistant against fixation and paraffin embedment. The monoclonal antibodies included were MT1, MT2, MB1, MB2, MB3, LN1, LN2, LN3, LeuM1, Leu7, VIE-G4, together with polyclonal antibodies reactive with immunoglobulin heavy and light chains, and with lysozyme and S100-protein. The preservation of morphological detail together with immunoperoxidase staining of cellular subsets, allowed an accurate determination of the ontogenic development of the different cell types in situ, in relation to their micro-environment. The use of paraffin tissue reactive (monoclonal) antibodies gives an extra dimension to the study of fetal lymphoid tissues. This is of particular advantage in studies on very fragile tissues as in early embryonal and fetal ontogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750398
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  • 2
    Electronic Resource
    Electronic Resource
    Haemopoiesis in human fetal and embryonic liver (1990)
    Springer
    Virchows Archiv 416 (1990), S. 429-436 
    Details
    ISSN: 1432-2307
    Keywords: Immunohistology ; Monoclonal antibodies ; MT1 ; Progenitor cells ; Haematopoiesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes and their microenvironment. Seven embryonal and twelve fetal liver specimens were studied, having been fixed in B5-fixative and embedded in paraffin. The different haemopoietic lineages each showed their own immunophenotype and distribution; intercellular and microenvironmental relationships were easily determined. Erythroid cells are reactive with VIE-G4, LN1, and MT1, sometimes partly surrounding a central macrophage. Myelomonocytic cells react with LCA, MT1, MB3, LN2, and anti-lysozyme, and from 14 weeks onwards with LN3. Lymphoid cells show LCA, MT1, MT2, MB1, MB2, MB3, and LN2 reactivity. In a few cases some scarce My10+ early progenitor cells were seen. An important finding is the extensive MT1-reactivity distributed over all haemopoietic lineages, and the demonstration of immature haemopoietic blast cells exclusively expressing the MT1 antigen. Further studies employing MT1 are necessary to delineate the extent of the distribution and the possible function of the antigen. Use of the MT1 mAb may contribute to the elucidation of the exact nature of the haemopoietic blast cells and their place in haemopoietic development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605149
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunogold labelling of leukemic hairy cells with the B-ly7 monoclonal antibody: An SEM and TEM study (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 356-367 
    Details
    ISSN: 1059-910X
    Keywords: Hairy cell leukemia ; Immunogold labelling ; B-ly7 antibody ; Electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Two cases of hairy cell leukemia have been studied by immuno-TEM and immuno-SEM after immunogold labelling of the cell surface antigen recognized by the B-ly7 monoclonal antibody.Most hairy cells appeared significantly labeled, although the density of the expression of the antigen, as demonstrated by immunogold labelling, seems variable from cell to cell. Moreover, some cells with the morphology of hairy cells and which could not be identified as monocytes were not labeled. Labelling for the antigen identified by the B-ly7 mAb does not seem to correlate with the presence of ribosome lamellae complexes which were present only in one of the two cases studied. Rare lymphocytes of unidentified lineage were labeled. Monocytes were significantly absent from the samples of peripheral blood of the two patients studied. In one normal control sample, monocytes were observed unlabelled.The results are discussed in reference to the pathogenesis of hairy cell leukemia, its surprisingly low mitotic rate, and its distinct response to chemotherapy. © 1994 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070280410
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    Paper (German National Licenses)
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