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  • Immunohistochemistry  (2)
  • Luteinizing hormone  (1)
  • Post-embedding method  (1)
  • Tissue culture  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immunoreactive luteinizing hormone in functioning corticotroph adenomas of the pituitary (1990)
    Springer
    Virchows Archiv 417 (1990), S. 361-367 
    Details
    ISSN: 1432-2307
    Keywords: Corticotroph adenoma ; Adrenocorticotropin ; Luteinizing hormone ; Immunohistochemistry ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two pituitary adenomas removed from a 37-year-old woman and a 26-year-old woman with typical Cushing's disease were studied by light and electron microscopy, immunohistochemistry and radioimmunoassay of tissue culture media. Both patients had high plasma levels of cortisol and normal levels of luteinizing hormone (LH). Both tumours were monomorphous, composed of densely granulated corticotrophs; the tumour cells contained periodic acid-Schiff positivity, were arranged in a sinusoidal pattern and, ultrastructurally, contained well-developed cytoplasmic organelles. By immunohistochemistry the majority of tumour cells contained immunoreactive adrenocorticotropin (ACTH); approximately 10% of the tumour cell population contained LH immunoreactivity. The LH-positive cells tended to form clusters scattered widely throughout the tumour tissues. LH immunoreactivity was demonstrated in some ACTH-immunoreactive cells on serial sections. Large amounts of immunoreactive ACTH and smaller quantities of LH, follicle stimulating hormone and αsubunit were released into the culture media and release of the glycoprotein hormones responded in parallel to corticotropin releasing hormone stimulation or inhibition by cortisol. These findings indicate that LH can be simultaneously produced and released by ACTH-producing tumour cells of otherwise typical functioning corticotroph adenomas. The capacity for LH production may be acquired during neoplastic proliferation. This is the first detailed report of concurrent production of LH by pituitary corticotroph adenomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605790
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Phenotypic modulation of smooth muscle cells in human cerebral aneurysmal walls (2000)
    Springer
    Acta neuropathologica 100 (2000), S. 475-480 
    Details
    ISSN: 1432-0533
    Keywords: Key words Cerebral aneurysm ; Immunohistochemistry ; Smooth muscle cell ; Phenotypic modulation ; Myosin heavy chain isoforms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We used immunohistochemical methods to analyze the phenotypes of smooth muscle cells (SMCs) in human cerebral arteries and aneurysmal walls. Thirty-two aneurysmal walls were studied; 31 aneurysmal walls were resected at operation and 1 aneurysm was obtained at autopsy. Seven control arteries were obtained at autopsy. Semiserial sections were subjected to immunohistochemical staining with antibodies to α-smooth muscle actin (α-SMA), desmin and smooth muscle myosin heavy chain isoforms: SM1, SM2 and SMemb. In control cerebral arteries, SMCs in the media were strongly immunostained for α-SMA, desmin, SM1 and SM2; immunoreactivity for SMemb was faint or weakly positive. SMCs in both non-ruptured and ruptured aneurysmal walls showed no staining for desmin; the expression of α-SMA was well preserved. Compared with control cerebral arteries, in 4 of 11 non-ruptured aneurysmal walls, the staining intensity of SMCs for SMemb was clearly increased. In ruptured aneurysmal walls, the expression of SM2 was lower than in control cerebral arteries and non-ruptured aneurysmal walls. Our study suggests that the phenotype of SMCs in aneurysmal walls is different from the contractile type in the media of normal cerebral arteries, at least partially changing to the synthetic type in some non-ruptured aneurysms. SMCs in ruptured aneurysmal walls may have lost both phenotypes before rupture. Phenotypic modulation of SMCs in the aneurysmal walls appears to be related to a remodeling of the aneurysmal wall and to a rupture mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010000220
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  • 3
    Electronic Resource
    Electronic Resource
    Use of ultrastructural immunohistochemistry in human pituitary pathology (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 20 (1992), S. 152-161 
    Details
    ISSN: 1059-910X
    Keywords: Pre-embedding method ; Post-embedding method ; Non-embedding method ; Protein A-gold technique ; Double labeling ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Recent advances in ultrastructural immunohistochemistry have provided insight into not only the subcellular localization of single antigens but also the colocalization of two distinct antigens in the same cellular constituent. In the field of pituitary pathology, precise identification of cell types, mechanism of processing, and dynamic intracellular transportation of hormones, as well as produciton of multiple hormones in the same cells of nontumorous and neoplastic adenohypophyses, have been documented by use of these techniques. The present review deals with the use of major methods for ultrastructural immunohistochemistry including pre-, post-, and non-embedding methods, paricularly focusing on their application to human pituitary pathology. Problems of tissue processing and a protocol for double labeling technique using the protein A-gold complex are also described.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070200204
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    Paper (German National Licenses)
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