ISSN:
1432-0711
Keywords:
Ultrastructural of human sperms
;
In-vitro fertilization
;
Sperm washing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Ultrastructural changes of human sperms after routine preparation for invitro fertilization were examined by scanning electron microscopy. Studies were performed with freshly ejaculated semen of 21 normozoospermic patients. Spermatozoa were analysed at 10000-fold (sperm head with acrosome, postacrosomal border and postnuclear cap) and 2500-fold (midpiece and endpiece of sperm tail) magnification. Compared with untreated specimens, slight membrane damage was found after routine washing and centrifugation procedures in swim-up preparations. However, on the basis of a score system for quantification of morphologic data, no statistically significant differences existed between untreated semen and swim-up preparations. We conclude that, with normozoospermic semen, the rate of ultrastructural damage attributable to sperm-washing procedures is too low to be of clinical consequence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02456677
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